The proteomics facility was established during 2008.
The facility was funded by DBT-CREBB
The mission of our Proteomics Facility is to provide protein analysis services along with consultation not only for University of Hyderabad community at large, but also for other academia, research organizations and industries. The facility routinely conducts training and workshops for faculty & students apart from refresher courses for degree college lecturers every year. Proteomics facility is equipped with state of the art mass spectrometers; MALDI TOF/TOF and ESI-LCMS Q-TOF.The facility is equipped with robust bioinformatics tools to identify and characterize proteins. The facility has been upgraded with the latest Surface Plasmon Resonance (SPR) system for protein-protein, protein-small molecules and protein nucleic acid interactions.
Our services include protein identification and protein quantitation from a wide variety of sample types, from simple mixtures (gel spots and bands) to complex mixtures (protein complexes, cell lysates, and plasma). Our principal approach for the analysis of proteins is 'bottom-up' proteomics, where all proteins are proteolytically digested, producing peptide surrogates (signature peptides) of the original proteins. Protein or peptide molecular weight determination by MALDI TOF/TOF or LCMS-ESI Q TOF.
Instruments available in Proteomics Facility:
MALDI TOF/TOF-AutoflexIII smart beam , Bruker Daltonics
ESI Q-TOF-LCMS/MS-microTOFq , Bruker Daltonics
Analytical HPLC, Agilent Technologies
Surface Plasmon Resonance SPR-Biacore T200, GE- Healthcare
Iso-electric focusing Ettan IPG phor3, GE- Healthcare
EttanDalt six vertical gel electrophoresis units with multi temp III cooling system and power supply, GE- Healthcare
MiniVE Vertical gel electrophoresis system, Amersham Biosciences
Hoefer SE 600 Ruby Vertical gel electrophoresis system Amersham Biosciences
Typhoon Trio + Variable mode Imager scanner, GE- Healthcare
Image Scanner for gels, GE- Healthcare
Ettan Spot Picker, Amersham Biosciences
Akta Pilot at Protein Purification Facility, GE- Healthcare
Filtration Unit at Protein Purification Facility, GE- Healthcare
Differential analysis DeCyder2D vr 7.0, GE- Healthcare
Image Master 2D Platinum IMP vr 6.0 and vr7.0 GE- Healthcare
IQTL vr7.0 GE- Healthcare
Biotools vr 3.1,Bruker Daltonics
Mascot Server (protein identification).