Faculty Details

Faculty Details

  • Name: Naresh Babu V. Sepuri
  • Mail: nbvssluohyd.ernet.in
  • Contact: 9959631608
  • Area Of Research:Mitochondrial biogenesis, retrograde signaling and redox homeostasis in health and disease using mammalian and yeast model systems

Prof Naresh Sepuri received his Ph.D. in 1997 from the University of Hyderabad in the area of Translational Regulation. Prof Sepuri carried out his post-doctoral studies at the University of Pennsylvania, Johns Hopkins University, and Thomas Jefferson University before joining the University of Hyderabad as Associate Professor in 2007. During his post-doctoral studies, he focused on protein translocation into mitochondria, mitochondrial biogenesis, and mitochondrial protein homeostasis. Prof Sepuri was a recipient of the American Heart Association, the USA during his post-doctoral work. He has 22 years of experience in the field of mitochondrial biology, of which 11 years were in the USA. He has numerous peer-reviewed publications in high impact international journals including Science, Molecular Cell, Journal of Cell Biology, and EMBO. Prof Sepuri's publications have been cited more than 2500 times by his peers that includes more than 1000 citations from the papers published with the University of Hyderabad affiliation. So far, Prof Sepuri has generated grant worth of INR 4.0 cr through funding from Government Agencies like DBT, DST, CSIR, ICMR, UGC, and an industry grant. He has reviewed papers from journals like Human Molecular Genetics, BBA, JBC, Mitochondrion, Science Signaling, Science Translational Research, Traffic and is also a Reviewer of the NSF grant (USA) and SERB (India). 

    Our lab focuses on a multi-dimensional and multipronged approach to understand the importance of mitochondrial redox homeostasis in viral infection, aging, cellular proliferation, metabolism, pathogenesis, and retrograde signaling using yeast, mammalian cell lines, and animal models. We would like to identify the components of the mitochondria acting as 'redox switches' in response to various stress conditions. The other aspect that we are trying to understand is the involvement of 'redox switches' in mitophagy, autophagy, and mitochondrial biogenesis. In this direction, we identified a couple of factors GrpEL1, a co-chaperone of mitoHsp70, and MIA40, an intermembrane space receptor that regulates the mitochondria protein and redox homeostasis. We also identified STAT3, a nuclear transcription factor is localized in the mitochondria and regulates respiration independent of transcription. These studies yielded high impact publications, including Science (2009), Mol. Biol. Cell (2013 & 15), JBC (2013), Biochemistry (2016), Biochem.J (2015), FEBs Letts (2020), JVI (2020). Further, deepen understanding of these and additional factors would strengthen our research activities and have a broader potential to understand the redox metabolism and to combat various disorders associated with mitochondrial dysfunction.

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Students/Projects Trainees

Name Joined As Mail Contact
ARPAN CHATTERJEE Ph.D STUDENT arpanchatterje1994@gmail.com ---
PRIYANKA ADLA Ph.D STUDENT priyankaa.adla@gmail.com ---
AMITA PAL Ph.D student amita.pal0320@gmail.com ---
VANDANA BISOYI Ph.D student bisoyi.vandana@gmail.com ---
ARUN KUMAR PARIPATI PhD Student arunreddy.paripati@gmail.com ---
YERRANNA BOGGULA Ph.D student yerranna.boggula@gmail.com ---
FAREED Md. Ph.D student fareed2406@gmail.com ---
USHODAYA MATTAM Post doctoral fellow mattamusha@gmai.com ---
32 Mohammed F, Gorla M, Bisoyi V, Tammineni P*, Sepuri NBV* (2020) , Rotenone-induced reactive oxygen species signal the recruitment of STAT3 to mitochondria , FEBS Letters, FEBS Press,doi: 10.1002/1873-3468.13741,,.
31 Chaitanya Gandikota, Fareed Mohammed, Lekha Gandhi, Deepti Maisnam, Ushodaya Mattam, Deepika Rathore, Arpan Chatterjee, Katyayani Mallick, Arcy Billoria, V S V Prasad, Naresh Babu Venkata Sepuri, Musturi Venkataramana (2020) , Mitochondrial import of dengue virus NS3 protease and cleavage of GrpEL1, a co-chaperone of mtHsp70; DOI: 10.1128/JVI.01178-20 , Journal of Virology, American Society for Microbiology,,, IF - 4.324.
30 Mohammed, Fareed., Gorla, Madhavi., Bisoyi, Vandana., Tammineni, Prasad., Sepuri, Naresh Babu V., (2020) , Rotenone‐induced reactive oxygen species signal the recruitment of STAT3 to mitochondria , FEBS letters, ,594,9,1403-1412.
29 Karri, Srinivasu., Singh, Swati., Paripati, Arun Kumar., Marada, Adinarayana., Krishnamoorthy, Thanuja., Guruprasad, Lalitha., Balasubramanian, Dorairajan., Sepuri, Naresh Babu V., (2019) , Adaptation of Mge1 to oxidative stress by local unfolding and altered interaction with mitochondrial Hsp70 and Mxr2 , Mitochondrion, Elsevier,46,,140-148.
28 Talari, Noble Kumar., Mattam, Ushodaya., Sepuri, Naresh Babu V., (2019) , Role of Mitochondria in Pancreatic Metabolism, Diabetes, and Cancer , Exploring Pancreatic Metabolism and Malignancy, Springer, Singapore,,,71-94.
27 Allu, Praveen Kumar., Boggula, Yerranna., Karri, Srinivasu., Marada, Adinarayana., Krishnamoorthy, Thanuja., Sepuri, Naresh Babu V., (2018) , A conserved R type Methionine Sulfoxide Reductase reverses oxidized GrpEL1/Mge1 to regulate Hsp70 chaperone cycle , Scientific reports, Nature Publishing Group,8,1,1-12.
26 Sepuri, Naresh BV., Angireddy, Rajesh., Srinivasan, Satish., Guha, Manti., Spear, Joseph., Lu, Bin., Anandatheerthavarada, Hindupur K., Suzuki, Carolyn K., Avadhani, Narayan G., (2017) , Mitochondrial LON protease-dependent degradation of cytochrome c oxidase subunits under hypoxia and myocardial ischemia , Biochimica et Biophysica Acta (BBA)-Bioenergetics, Elsevier,1858,7,519-528.
25 Kumari, Jyoti., Hussain, Mansoor., De, Siddharth., Chandra, Suruchika., Modi, Priyanka., Tikoo, Shweta., Singh, Archana., Sagar, Chandrasekhar., Sepuri, Naresh Babu V., Sengupta, Sagar., (2016) , Mitochondrial functions of RECQL4 are required for the prevention of aerobic glycolysis-dependent cell invasion , Journal of Cell Science, The Company of Biologists Ltd,129,7,1312-1318.
24 Marada, Adinarayana., Karri, Srinivasu., Singh, Swati., Allu, Praveen Kumar., Boggula, Yerranna., Krishnamoorthy, Thanuja., Guruprasad, Lalitha., V Sepuri, Naresh Babu., (2016) , A single point mutation in mitochondrial Hsp70 cochaperone Mge1 gains thermal stability and resistance , Biochemistry, American Chemical Society,55,51,7065-7072.
23 Allu, Praveen Kumar., Marada, Adinarayana., Boggula, Yerranna., Karri, Srinivasu., Krishnamoorthy, Thanuja., Sepuri, Naresh Babu V., (2015) , Methionine sulfoxide reductase 2 reversibly regulates Mge1, a cochaperone of mitochondrial Hsp70, during oxidative stress , Molecular biology of the cell, The American Society for Cell Biology,26,3,406-419.
22 Murari, Anjaneyulu., Thiriveedi, Venkata Ramana., Mohammad, Fareed., Vengaldas, Viswamithra., Gorla, Madhavi., Tammineni, Prasad., Krishnamoorthy, Thanuja., Sepuri, Naresh Babu V., (2015) , Human mitochondrial MIA40 (CHCHD4) is a component of the Fe–S cluster export machinery , Biochemical Journal, Portland Press Ltd.,471,2,231-241.
21 Gorla, Madhavi., Sepuri, Naresh Babu V., (2014) , Perturbation of apoptosis upon binding of tRNA to the heme domain of cytochrome c , Apoptosis, Springer US,19,1,259-268.
20 Saikia, Mridusmita., Jobava, Raul., Parisien, Marc., Putnam, Andrea., Krokowski, Dawid., Gao, Xing-Huang., Guan, Bo-Jhih., Yuan, Yiyuan., Jankowsky, Eckhard., Feng, Zhaoyang., (2014) , Angiogenin-cleaved tRNA halves interact with cytochrome c, protecting cells from apoptosis during osmotic stress , Molecular and cellular biology, American Society for Microbiology Journals,34,13,2450-2463.
19 Tammineni, Prasad., Anugula, Chandrashekhar., Mohammed, Fareed., Anjaneyulu, Murari., Larner, Andrew C., Sepuri, Naresh Babu Venkata., (2013) , The import of the transcription factor STAT3 into mitochondria depends on GRIM-19, a component of the electron transport chain , Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology,288,7,4723-4732.
18 Marada, Adinarayana., Allu, Praveen Kumar., Murari, Anjaneyulu., PullaReddy, BhoomiReddy., Tammineni, Prasad., Thiriveedi, Venkata Ramana., Danduprolu, Jayasree., Sepuri, Naresh Babu V., (2013) , Mge1, a nucleotide exchange factor of Hsp70, acts as an oxidative sensor to regulate mitochondrial Hsp70 function , Molecular biology of the cell, The American Society for Cell Biology,24,6,692-703.
17 Sepuri, Naresh Babu V., Gorla, Madhavi., King, Michael P., (2012) , Mitochondrial lysyl-tRNA synthetase independent import of tRNA lysine into yeast mitochondria , PloS one, Public Library of Science,7,4,e35321.
16 Bansal, Seema., Liu, Chuan-Peng., Sepuri, Naresh BV., Anandatheerthavarada, Hindupur K., Selvaraj, Venkatesh., Hoek, Jan., Milne, Ginger L., Guengerich, F Peter., Avadhani, Narayan G., (2010) , Mitochondria-targeted cytochrome P450 2E1 induces oxidative damage and augments alcohol-mediated oxidative stress , Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology,285,32,24609-24619.
15 Wegrzyn, Joanna., Potla, Ramesh., Chwae, Yong-Joon., Sepuri, Naresh BV., Zhang, Qifang., Koeck, Thomas., Derecka, Marta., Szczepanek, Karol., Szelag, Magdalena., Gornicka, Agnieszka., (2009) , Function of mitochondrial Stat3 in cellular respiration , Science, American Association for the Advancement of Science,323,5915,793-797.
14 Fang, Ji-Kang., Prabu, Subbuswamy K., Sepuri, Naresh B., Raza, Haider., Anandatheerthavarada, Hindupur K., Galati, Domenico., Spear, Joseph., Avadhani, Narayan G., (2007) , Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion , FEBS letters, ,581,7,1302-1310.
13 Sepuri, Naresh BV., Yadav, Sanjay., Anandatheerthavarada, Hindupur K., Avadhani, Narayan G., (2007) , Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N‐terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae− role of protein kinase A in the mitochondrial targeting of CYP2E1 , The FEBS Journal, Blackwell Publishing Ltd Oxford, UK,274,17,4615-4630.
12 Robin, Marie-Anne., Anandatheerthavarada, Hindupur K., Biswas, Gopa., Sepuri, Naresh Babu V., Gordon, Donna M., Pain, Debkumar., Avadhani, Narayan G., (2002) , Bimodal targeting of microsomal CYP2E1 to mitochondria through activation of an N-terminal chimeric signal by cAMP-mediated phosphorylation , Journal of Biological Chemistry, ASBMB,277,43,40583-40593.
11 Kovermann, Peter., Truscott, Kaye N., Guiard, Bernard., Rehling, Peter., Sepuri, Naresh B., Müller, Hanne., Jensen, Robert E., Wagner, Richard., Pfanner, Nikolaus., (2002) , Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel , Molecular cell, Cell Press,9,2,363-373.
10 Kerscher, Oliver., Sepuri, Naresh B., Jensen, Robert E., (2000) , Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane , Molecular Biology of the Cell, The American Society for Cell Biology,11,1,103-116.
9 Davis, Alison J., Sepuri, Naresh B., Holder, Jason., Johnson, Arthur E., Jensen, Robert E., (2000) , Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria , The Journal of cell biology, The Rockefeller University Press,150,6,1271-1282.
8 Anandatheerthavarada, Hindupur K., Biswas, Gopa., Mullick, Jayati., Sepuri, Naresh Babu V., Otvos, Laszlo., Pain, Debkumar., Avadhani, Narayan G., (1999) , Dual targeting of cytochrome P4502B1 to endoplasmic reticulum and mitochondria involves a novel signal activation by cyclic AMP‐dependent phosphorylation at ser128 , The EMBO journal, John Wiley & Sons, Ltd,18,20,5494-5504.
7 Schülke, Norbert., Sepuri, Naresh Babu V., Gordon, Donna M., Saxena, Sandeep., Dancis, Andrew., Pain, Debkumar., (1999) , A multisubunit complex of outer and inner mitochondrial membrane protein translocases stabilized in vivo by translocation intermediates , Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology,274,32,22847-22854.
6 Arakane, Futoshi., Kallen, Caleb B., Watari, Hidemichi., Foster, James A., Sepuri, Naresh Babu V., Pain, Debkumar., Stayrook, Steven E., Lewis, Mitchell., Gerton, George L., Strauss, Jerome F., (1998) , The mechanism of action of steroidogenic acute regulatory protein (StAR) StAR acts on the outside of mitochondria to stimulate steroidogenesis , Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology,273,26,16339-16345.
5 Knight, Simon AB., Sepuri, Naresh Babu V., Pain, Debkumar., Dancis, Andrew., (1998) , Mt-Hsp70 homolog, Ssc2p, required for maturation of yeast frataxin and mitochondrial iron homeostasis , Journal of Biological Chemistry, ASBMB,273,29,18389-18393.
4 Sepuri, Naresh Babu V., Gordon, Donna M., Pain, Debkumar., (1998) , A GTP-dependent “push” is generally required for efficient protein translocation across the mitochondrial inner membrane into the matrix , Journal of Biological Chemistry, ASBMB,273,33,20941-20950.
3 Sepuri, Naresh Babu V., Schülke, Norbert., Pain, Debkumar., (1998) , GTP hydrolysis is essential for protein import into the mitochondrial matrix , Journal of Biological Chemistry, ASBMB,273,3,1420-1424.
2 Schülke, Norbert., Sepuri, Naresh Babu V., Pain, Debkumar., (1997) , In vivo zippering of inner and outer mitochondrial membranes by a stable translocation intermediate , Proceedings of the National Academy of Sciences, National Academy of Sciences,94,14,7314-7319.
1 Babu, Sepuri V Naresh., Ramaiah, Kolluru V Atchuta., (1996) , Type 1 phosphatase inhibitors reduce the restoration of guanine nucleotide exchange activity of eukaryotic initiation factor 2B in inhibited reticulocyte lysates rescued by hemin , Archives of biochemistry and biophysics, Academic Press,327,2,201-208.
Title Year Duration Amount(In Lakhs) PI/I
Host-virus interactions - MHRD-IISC STARS 2019 3 990000000000 Dr. M. Venkataramana/ Co PI- Prof. Naresh Babu V. Sepuri, Department of Biochemistry