My lab is themed on ubiquitin signaling and genome editing. I am interested in identifying deubiquitinases crucial in important biological process and identify inhibitors against such deubiquitinases. We apply individual depletion strategy to members of Ubiquitin Proteasome System to identify crucial regulators of cellular processes and disease. Many of these proteins have distinct catalytic pockets, which can be targeted for inhibition by small molecule inhibitors. Such small molecule inhibitors can be developed into effective drugs. I am also interested in studying gene function in animal models using gene-editing technologies. CRISPR-based endogenous gene tagging allows studying gene functions at physiological relevant conditions. We use these technologies along with proteomics studies to elucidate functions of target genes.
|11||Ajay Chatrath, Roza Przanowska, Shashi Kiran, Zhangli Su, Shekhar Saha, Briana Wilson, Takaaki Tsunematsu, Ji-Hye Ahn, Kyung Yong Lee, Teressa Paulsen, Ewelina Sobierajska, Manjari Kiran, Xiwei Tang, Tianxi Li, Pankaj Kumar, Aakrosh Ratan, Anindya Dutta., (2020) , The pan-cancer landscape of prognostic germline variants in 10,582 patients , Genome Medicine, Springer Nature,12,15,.|
|10||Kumar, Pankaj., Kiran, Shashi., Saha, Shekhar., Su, Zhangli., Paulsen, Teressa., Chatrath, Ajay., Shibata, Yoshiyuki., Shibata, Etsuko., Dutta, Anindya., (2020) , ATAC-seq identifies thousands of extrachromosomal circular DNA in cancer and cell lines , Science Advances, American Association for the Advancement of Science,6,20,eaba2489.|
|9||Kiran, Shashi., Dar, Ashraf., Singh, Samarendra K., Lee, Kyung Yong., Dutta, Anindya., (2018) , The deubiquitinase USP46 is essential for proliferation and tumor growth of HPV-transformed cancers , Molecular cell, Cell Press,72,5,823-835. e5.|
|8||Shibata, Etsuko., Kiran, Manjari., Shibata, Yoshiyuki., Singh, Samarendra., Kiran, Shashi., Dutta, Anindya., (2016) , Two subunits of human ORC are dispensable for DNA replication and proliferation , Elife, eLife Sciences Publications Limited,5,,e19084.|
|7||Kiran, Shashi., Oddi, Vineesha., Ramakrishna, Gayatri., (2015) , Sirtuin 7 promotes cellular survival following genomic stress by attenuation of DNA damage, SAPK activation and p53 response , Experimental cell research, Academic Press,331,1,123-141.|
|6||Kiran, Shashi., Anwar, Tarique., Kiran, Manjari., Ramakrishna, Gayatri., (2015) , Sirtuin 7 in cell proliferation, stress and disease: Rise of the Seventh Sirtuin! , Cellular signalling, Pergamon,27,3,673-682.|
|5||Singh, Sapna., Kumar, P Uday., Thakur, Suresh., Kiran, Shashi., Sen, Bijoya., Sharma, Shreya., Rao, Vishnu Vardhan., Poongothai, AR., Ramakrishna, Gayatri., (2015) , Expression/localization patterns of sirtuins (SIRT1, SIRT2, and SIRT7) during progression of cervical cancer and effects of sirtuin inhibitors on growth of cervical cancer cells , Tumor Biology, Springer Netherlands,36,8,6159-6171.|
|4||Kiran, Shashi., Chatterjee, Nirupama., Singh, Sapna., Kaul, Sunil C., Wadhwa, Renu., Ramakrishna, Gayatri., (2013) , Intracellular distribution of human SIRT 7 and mapping of the nuclear/nucleolar localization signal , The FEBS journal, ,280,14,3451-3466.|
|3||Ramakrishna, Gayatri., Anwar, Tarique., Angara, Rajendra Kumar., Chatterjee, Nirupama., Kiran, Shashi., Singh, Sapna., (2012) , Role of cellular senescence in hepatic wound healing and carcinogenesis , European journal of cell biology, Urban & Fischer,91,10,739-747.|
|2||Chatterjee, Nirupama., Kiran, Shashi., Ram, Babul Moni., Islam, Nashreen., Ramasarma, Tangirala., Ramakrishna, Gayatri., (2011) , Diperoxovanadate can substitute for H2O2 at much lower concentration in inducing features of premature cellular senescence in mouse fibroblasts (NIH3T3) , Mechanisms of ageing and development, Elsevier,,,.|
|1||Kiran, Shashi., Batra, Munish., Kumar, Umapati V Jitentdra., Saxena, MK., (2009) , Detection of virulence in Salmonella Typhimurium isolated from Ganga water , International Journal of Applied Environmental Sciences, Research India Publications,4,4,363-367.|
|Characterization of the E6-USP46-Cdt2 interaction and epigenetic role of, subsequent Set8 degradation, in HPV induced cancers||2020||2 years||10||Shashi Kiran|
|Exploiting E6-USP46 Deubiquitinase Complex, As A Novel Therapeutic Target Against Human Papilloma Virus (HPV) Induced Cancers.||2020||3 years||30||Shashi Kiran|
Feb, 2020 - Current- Assistant Professor, Department of Biochemistry, School of Life Sciences, University of Hyderabad.
Sep,2014-Jan,2020 : Research Associate, School of Medicine, University of Virginia.
Jan,2008 - Oct,2014 : PhD, Center for DNA Fingurprinting and Diagnostics (CDFD)/ University of Hyderabad.
2005-2007- M.V.Sc (Animal Biotechnology), G.B.Pant University of Agriculture and Technology (GBPUA&T)
2000-2005- B.V.Sc & A.H. (Veterinary Sciences and Animal Husbandry) , Central Agricultuarl University