Central Facilities

Description

The Genomics Facility at School of Life Sciences, University of Hyderabad, is an important core facility of the School. It provides infrastructure and service support for research needs of scientists in the area of high-throughput genomics. We provide the following services in the facility on payment basis: Sample Preps: DNA and RNA extractions, Plasmid preps. QC for RNA, DNA, and High-throughput Sequencing Libraries. Real-time PCR and gene expression, genotyping, melt curves, etc. Microarray Gene Expression (Agilent platform). Eukaryote Cell Transfection & Prokaryote cell Transformation (BTX Gemini X2 System). Bioluminescence Assays using Luminometere (Promega GloMax-96 microplate). Access to Instrumentation and Software. Electroporation System, BTX Gemini Systems. Focused – Ultra Sonicator, Covaris M220. Light Cycler 480II, Roche. The Genomics Facility provides services for gene expression and genotyping studies utilizing microarray and real time PCR, and related services to researchers within the University and beyond. The facility also provides access to instruments, equipment and software utilized within the microarray field and supports Agilent and other microarray platforms. In addition, the facility can also print custom microarrays utilizing cDNA, oligonucliotides, proteins, peptides, antibodies, cell lysates, siRNAs, and other types of biological materials. This facility has been used regularly for past eight years to impart training to students, post docs and junior faculty within UoH School of Life Sciences (SLS-UoH). More importantly, the facility has significantly improved the research output from UoH during the past few years. However, an important mandate of this infrastructure is training of junior and mid-career faculty from State universities and colleges. We have already conducted more than 50 workshops targeting State University faculty during past 5 years. The workshops not only included hands on training for UGC staff college participants which were primarily lecturers/faculty from several State Government Universities but also training and providing research support to international students who regularly visit UOH under exchange programs such as IRTG, DAAD, etc. Thus the facility has successfully achieved its mandate to: Disseminate technology related to new developments in the field of genomics. Ensure improvement of skills and teaching qualities in state universities and colleges by imparting theoretical and practical training to teachers. To facilitate young researchers in state universities/ colleges to commence important research projects of relevance to their respective state/Union territories.

Description

The proteomics facility was established during 2008. The facility was funded by DBT-CREBB Mission: The mission of our Proteomics Facility is to provide protein analysis services along with consultation not only for University of Hyderabad community at large, but also for other academia, research organizations and industries. The facility routinely conducts training and workshops for faculty & students apart from refresher courses for degree college lecturers every year. Proteomics facility is equipped with state of the art mass spectrometers; MALDI TOF/TOF and ESI-LCMS Q-TOF.The facility is equipped with robust bioinformatics tools to identify and characterize proteins. The facility has been upgraded with the latest Surface Plasmon Resonance (SPR) system for protein-protein, protein-small molecules and protein nucleic acid interactions. Services offered: Our services include protein identification and protein quantitation from a wide variety of sample types, from simple mixtures (gel spots and bands) to complex mixtures (protein complexes, cell lysates, and plasma). Our principal approach for the analysis of proteins is ‘bottom-up’ proteomics, where all proteins are proteolytically digested, producing peptide surrogates (signature peptides) of the original proteins. Protein or peptide molecular weight determination by MALDI TOF/TOF or LCMS-ESI Q TOF. Instruments available in Proteomics Facility: MALDI TOF/TOF-AutoflexIII smart beam , Bruker Daltonics ESI Q-TOF-LCMS/MS-microTOFq , Bruker Daltonics Analytical HPLC, Agilent Technologies Surface Plasmon Resonance SPR-Biacore T200, GE- Healthcare Iso-electric focusing Ettan IPG phor3, GE- Healthcare EttanDalt six vertical gel electrophoresis units with multi temp III cooling system and power supply, GE- Healthcare MiniVE Vertical gel electrophoresis system, Amersham Biosciences Hoefer SE 600 Ruby Vertical gel electrophoresis system Amersham Biosciences Typhoon Trio + Variable mode Imager scanner, GE- Healthcare Image Scanner for gels, GE- Healthcare Ettan Spot Picker, Amersham Biosciences Akta Pilot at Protein Purification Facility, GE- Healthcare Filtration Unit at Protein Purification Facility, GE- Healthcare Software available: Differential analysis DeCyder2D vr 7.0, GE- Healthcare Image Master 2D Platinum IMP vr 6.0 and vr7.0 GE- Healthcare IQTL vr7.0 GE- Healthcare Biotools vr 3.1,Bruker Daltonics Mascot Server (protein identification).

Description

List of the instruments at Metabolomics Facility LC-MS/MS Q-Tof GC-MS Liquid Chromatography Mass Spectrometer Instrument Details Make : Agilent technologies, USA Model : 6520-Accurate Mass Q-TOF LC/MS. Specification : Direct Infusion Mass with ESI Positive & Negative mode ionization, mass ranging from 50 to 3200 m/z HPLC PDA Detector -Mass spectrometer Working Principles: Liquid chromatography / Mass Spectroscopy (LC / MS) is a technique which combines high performance liquid chromatography HPLC, a powerful analytical separation technique with mass spectrometry, a powerful analysis & detection technique. There is a common atmospheric pressure ionization (API) LC/MS process: Electrospray Ionization (ESI). API processes are also compatible with most mobile phase solvents (volatile) & volatile buffers. Main Features of the Instrument: Mass Range: 50-3200 m/z Resolution: 20000 FWHM High Mass Accuracy (Typically less than 2ppm) High Sensitivity Ionization Method : ESI positive & negative Direct Infusion for Mass Analysis (MS, MS /MS) MS/MS function: A. Sample Injection B. Ionization C. Fragmentation (CID) D. Ions travel through Quadruple and Time flight tube and reach the detector on the basis of their m/z value. Applications Qualitative Analysis Quantitative Analysis Impurity Profiling Metabolite Studies Pharmacokinetics Pharma & biomedical applications: Gas Chromatograph with high resolution Mass Spectrometer (GC-HRMS) Instrument Details Make of MS : LECO Model : Pegasus HT TOF MS Specifications : EI Source Time of Flight Analyzer Mass range -50 – 1000 amu Mass resolution – 1000 Make of GC : Agilent: 7890 A gas-chromatograph coupled with mass spectrometer (GC-MS) is a combined analyzer that has a superior ability in analyzing organic compounds qualitatively and quantitatively. User Instructions: Expected accurate mass can be provided. Solvent for mobile phase should be mentioned or solubility of compound in volatile solvent should be given. At present C8, C18 & others like cyano, amino, Si columns are available. You can submit the column of your interest for separation or repeatability of HP-LC run. Source of the compound should be mentioned. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there are no toxic sample being given. Samples should not be toxic or hazardous. Samples will not be accepted unless accompanied by MS-DS. User Charges: User Charges of Metabolomics Facility: LC-MS /MS-Q-TOF, LC-MS/MS (ESI): Students of UOH: Rs.1500/- per sample. Students from other educational institutions: Rs.3000/- per sample. Persons from Industries: Rs.4500/- per sample. GC-MS, Library:NIST, GC_MS/MS Students of UOH: Rs.1000/- per sample. Students from other educational institutions: Rs.2000/- per sample. Persons from Industries: Rs.4000/- per sample.

Description

BSL-3 facility at University of Hyderabad for research on pathogens: 1.Brucella melitensis, Brucella abortus 2.Pathogenic Mycobacteria BSL – 3 Main Lab 1.Separated from areas that are open to unrestricted traffic flow within the building with limited access. 2.Maintained at -55pa. 3.The air is discharged through HEPA filters which are capable of filtering 0.3 micron air-borne particles with an efficiency of 99.97%. 4.The biohazard waste generated from the lab is directly sent to the autoclave via a different pass box provided. Animal BSL – 3 Lab 1.The ABSL-3 is equipped with “Individually ventilated cages” (IVC) with virus filtration efficiency ?99.999987% and bacteria filtration efficiency ?99.9999937%. 2.Also ensures optimal gas exchange as well as protection also during procedures or in case of power failure. 3.ABSL-3 lab is maintained at -52pa. 4.ABSL-3 lab also has a challenge room or dissection room for the challenge studies. Each room in the ABSL-3 lab is connected by pass box for the passage of the experimental samples. Pre-culture Room Both BSL-3 main lab and ABSL-3 lab is connected by pass box for the passage of the experimental samples. Well equipped decontamination facility.

Description

The flow Cytometry facility at the Department of Animal Biology was established with funds provided under DST-FIST program. The facility houses three FACS instruments namely:- 1.Bench top BD LSR Fortessa Flow Cytometry system 2.Bench top BD FACS Aria-III System and 3.FACS Calibur Flow Cytometry system This facility is open for all the research across the university, other academic research institutes and industries and the instruments are being used extensively FLOW CYTOMETRY (FACS) FACILITY The flow Cytometry facility at the Department of Animal Biology was established with funds provided under DST-FIST program. The facility houses three FACS instruments namely:- Bench top BD LSR Fortessa Flow Cytometry system Bench top BD FACS Aria-III System and FACS Calibur Flow Cytometry system This facility is open for all the research across the university, other academic research institutes and industries and the instruments are being used extensively img User Charges FACS Acquisition Service Booking: 1.For users of University of Hyderabad:Rs.500/- per Hour 2.For users of other Academic & research institutions:Rs.1000/-per Hour 3.For users from Industries:Rs.1500/- per Hour

• Faculty In-charge: Roy Karnati
• Mail:roykarnati@gmail.com
• Contact No:

Description

Company: Carl Zeiss Model: NLO 710 Software: ZEN 2010 Laser attached (absorption in nm): 458,488,543,633 and multi-photon laser (780-1000 nm). Studies Undertaken: Localization, Co-localization, Staining properties, Z-Scan, 3D image development, Optical sectioning, FRAP studies, FRET studies and Lambda detection. Samples worked with: Fluorescent stained, auto fluorescent animal and plant cell lines (both fixed and suspensions). Tissue sections, whole brain mounts of insects, powders and drop castings of chemical compounds, fluorescent studies of Laser etched metals and powder compounds. User Charges: Charges based on number of slots used where one and a half hour is considered as one slot. Students of UOH: Rs.500/- per slot. Students from other educational institutions: Rs.1000/- per slot. Persons from companies: Rs.2000/- per slot. Note: Prior booking slots is mandatory by the user.

Description

Equipment Name: In Vivo Imaging System

Model: Kodak System; Aquired by Carestream Health Inc, USA and now with Bruker, Germany.

Disrtibutor: InKarp Instruments Pvt Ltd.

Light Source:400 W Xenon.

Monochrome interlined, fixed lens(10x), cooled (-29 C, absolute) CCD camera (13.8 x 13.8 cm/ 2048 x 2048 px, 67 umpx, 16bit)

Imaging Modelities: Bioluminescence, Multi-wavelength fluorescence, X-ray, Reflected Light, Radioisotopic

Excitation Filters: 28 automated bandpass excitation filtersavailable (410 nm – 760 nm)

Emission Filters: 6 automated wide angle emission filters availab;e (535 nm -830 nm)

Isoflurane vaporizer for anesthesia during imaging

Animal warming (20oC – 40oC) for longer-term anesthesia

Computer command center running Windows 7 Pro with Carestream MI acquisition and analysis software

User Charges:

User Charges of In-Vivo Imaging Facility:

Live Imaging without expensive chemicals:

Students of UOH: Rs.250/- per hour.

Students from other educational institutions: Rs.500/- per hour.

Persons from companies: Rs.1000/- per hour.

Live Imaging with expensive chemicals:

Students of UOH: Rs.1000/- per hour.

Students from other educational institutions: Rs.2000/- per hour.

Persons from companies: Rs.4000/- per hour.

• Faculty In-charge:DEAN
• Mail:deansls@uohyd.ac.in
• Contact No:

Description

The Auditorium and Seminar Hall should be exclusively used for academic conferences and scientific meetings, and will not be allowed, under any circumstances, for any other purpose, including training programmes, faculty/student gatherings. Booking will be done on priority basis. However, if any adjustments are required, they will be made based on priority of the events. Booking will be taken care of the Dean’s Office in consultation with the Auditorium & Seminar Hall Committee. Booking will be accepted only after the payment of user charges in advance. The Faculty /Staff who booked the facility would be responsible for the upkeep of the facility during the period (including equipment’s of Auditorium and Seminar Hall.) At any given time, the booking will be done for two to four days only. No food/drinks will be allowed into the Auditorium and Seminar Hall. User Charges The committee recommended the following user charges for the Auditorium and Seminar Hall. Auditorium Rs. 3000/- per day for faculty of Life Sciences. Rs. 5000/- per day faculty of non-Life Sciences, but under UoH. For all other events and conferences sponsored by other funding bodies, Rs. 10,000/- per day will be charged. Seminar Hall For Pre-Ph.D. seminars for SLS: Rs. 500/- for two hours. For visiting faculty/scientist talks/colloquiums of Life Sciences: Rs. 2000/- per day For faculty of non-Life Sciences and Schools of University: Rs. 2,500/- per day, and For all other events and conferences, sponsored by other funding bodies, Rs. 7,000/- per day will be charged.

Description

The Bioinformatics Infrastructure Facility (BIF) was established in this university in the year 2006 with the financial support of the Department of Biotechnology, Government of India under the Biotechnology Information System Network (BTISnet) programme. The primary objective of this facility is to promote biology teaching through bioinformatics (BTBI).

This is an University facility for Biocomputing, wherein School of Life Sciences is a nodal School in management of the facility.The aim of the facility is to provide Biocomputing infrastructure for researchers in various disciplines of Science and Technology for development of innovative methodologies, techniques, tools. Further, the facility augments research in the area of Systems Biology and Drug Discovery.

Facility supports the computational requirement of doctoral and PG students for carrying out thesis / project work. Further, students from various public funded institutions are selected periodically for training in the area of Drug Discovery.

The activities of the facility are coordinated by the Coordinating Committee with following members:

Coordinator

Prof. H.A.Nagarajaram

Dept. of Systems and Computational Biology

School of Life Sciences,UoHatics

Co-coordinators

Prof. Lalitha Guruprasad, SC

Dr. S. Durga Bhavani, SCIS

Dr. S. Rajagopal, PS, SLS 

Dr. K. Sreenivasulu, AS, SLS 

Dr. P. Anil Kumar, BC, SLS      

Development of bioinformatics tools with applications in Drug Design and Systems Biology

Development of new databases based on the gene structure, transcription activity and function

Creation of integrated platform like HIV Information Resource.

Training maters and doctoral students in Drug design and systems biology

• Faculty In-charge: H.A.Nagarajaram
• Mail:hansl@uohyd.ac.in
• Contact No:040-23134561