Faculty Details

Faculty Details

  • Name: Dr. Jogi Madhuprakash
  • Mail: jmpsluohyd.ac.in
  • Contact: 040-23134566
  • Area Of Research:CAZymes, Protein Engineering and Proteomics
Our group works mainly on enzyme discovery, characterization, engineering and application of proteins and enzymes which are involved primarily in the lignocellulose or chitin biomass degradation and modification. We aim at combining high-level basic research with applied studies and innovation. Together with the basic characterization of proteins and enzymes, we use advanced enzyme engineering technologies and state-of-the art analytical, structural and biophysical techniques to obtain an in-depth knowledge about the target proteins or enzymes. This will provide a clear platform for an efficient use of recalcitrant polymers like cellulose or chitin and to generate products-of-value in a sustainable biorefinery. Furthermore, we employ several molecular techniques for functional validation of the enzymatically generated products with a focus on improvement of plant health.
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Students/Projects Trainees

Name Joined As Mail Contact
Saumashish Mukherjee Ph.D. saumashish.13sm@outlook.com ---
Koyel Bardhan Ph.D. koyelbardhan@gmail.com ---
Lalduhsaki T Ph.D. lalduhsaki29@gmail.com ---
Vandhana T M Ph.D. vandhanamadhusoodhanan@gmail.com ---
N. Akshay Ph.D. akyavy@gmail.com ---
29 Vandhana, Theruvothu Madathil., Reyre, Jean-Lou., Sushmaa, Dangudubiyyam., Berrin, Jean-Guy., Bissaro, Bastien., Madhuprakash, Jogi. (2022) , On the expansion of biological functions of lytic polysaccharide monooxygenases , New Phytologist, WILEY,,,.
28 Gopalakrishnan, Subramaniam., Srinivas, Vadlamudi., Naresh, Nimmala., Pratyusha, Sambangi., Ankati, Sravani., Madhuprakash, Jogi., Govindaraj, Mahalingam., Sharma, Rajan., (2021) , Deciphering the antagonistic effect of Streptomyces spp. and host-plant resistance induction against charcoal rot of sorghum , Planta, Springer,253,2,1-12.
27 Kumar, Manish., Madhuprakash, Jogi., Balan, Venkatesh., Singh, Amit Kumar., Vivekanand, V., Conceptulization, Nidhi Pareek., (2021) , Chemoenzymatic production of chitooligosaccharides employing ionic liquids and Thermomyces lanuginosus chitinase , Bioresource Technology, Elsevier,,,125399.
26 Duhsaki, Lal., Mukherjee, Saumashish., Rani, Tirupaati Swaroopa., Madhuprakash, Jogi., (2021) , Genome analysis of Streptomyces sp. UH6 revealed the presence of potential chitinolytic machinery crucial for chitosan production , Environmental Microbiology Reports, John Wiley & Sons, Inc. Hoboken, USA,,,.
25 Rani, Tirupaati Swaroopa., Nadendla, Sandhya Rani., Bardhan, Koyel., Madhuprakash, Jogi., Podile, Appa Rao., (2020) , Chitosan conjugates, microspheres, and nanoparticles with potential agrochemical activity , Agrochemicals Detection, Treatment and Remediation, Elsevier,,,437-464.
24 Madhuprakash, Jogi., Rani, T Swaroopa., Podile, Appa Rao., Eijsink, Vincent GH., Sørlie, Morten., (2020) , Thermodynamic insights into the role of aromatic residues in chitooligosaccharide binding to the transglycosylating chitinase-D from Serratia proteamaculans. , Biochimica et Biophysica Acta. Proteins and proteomics, ,1868,7,140414.
23 Rani, T Swaroopa., Madhuprakash, Jogi., Podile, Appa Rao., (2020) , Chitinase-E from Chitiniphilus shinanonensis generates chitobiose from chitin flakes , International Journal of Biological Macromolecules, Elsevier,163,,1037-1043.
22 Mukherjee, Saumashish., Behera, Prashanta Kumar., Madhuprakash, Jogi., (2020) , Efficient conversion of crystalline chitin to N-acetylglucosamine and N, N\'-diacetylchitobiose by the enzyme cocktail produced by Paenibacillus sp. LS1 , Carbohydrate Polymers, Elsevier,250,,116889.
21 Harmsen, Rianne AG., Aam, Berit Bjugan., Madhuprakash, Jogi., Hamre, Anne Grethe., Goddard-Borger, Ethan D., Withers, Stephen G., Eijsink, Vincent GH., Sørlie, Morten., (2020) , Chemoenzymatic Synthesis of Chito-oligosaccharides with Alternating N-d-Acetylglucosamine and d-Glucosamine , Biochemistry, American Chemical Society,59,,4581−4590.
20 Manjeet, Kaur., Madhuprakash, Jogi., Mormann, Michael., Moerschbacher, Bruno M., Podile, Appa Rao., (2019) , A carbohydrate binding module-5 is essential for oxidative cleavage of chitin by a multi-modular lytic polysaccharide monooxygenase from Bacillus thuringiensis serovar kurstaki , International Journal of Biological Macromolecules, Elsevier,127,,649–656.
19 Madhuprakash, Jogi., Dalhus, Bjørn., Vaaje-Kolstad, Gustav., Sakuda, Shohei., Podile, Appa Rao., Eijsink, Vincent GH., Sørlie, Morten., (2019) , Structural and Thermodynamic Signatures of Ligand Binding to the Enigmatic Chitinase D of Serratia proteamaculans , The Journal of Physical Chemistry B, American Chemical Society,123,10,2270-2279.
18 Bhuvanachandra, Bhoopal., Madhuprakash, Jogi., Podile, Appa Rao., (2018) , Active-site mutations improved the transglycosylation activity of Stenotrophomonas maltophilia chitinase A , Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, Elsevier,1866,3,407-414.
17 Madhuprakash, Jogi., Dalhus, Bjørn., Rani, T Swaroopa., Podile, Appa Rao., Eijsink, Vincent GH., Sørlie, Morten., (2018) , Key residues affecting transglycosylation activity in family 18 chitinases: insights into donor and acceptor subsites , Biochemistry, American Chemical Society,57,29,4325-4337.
16 Kalyani, Dayanand., Madhuprakash, Jogi., Horn, Svein Jarle., (2017) , Laccases: Blue Copper Oxidase in Lignocellulose Processing , Microbial Applications, Springer,2,,315-336.
15 Vaikuntapu, Papa Rao., Rambabu, Samudrala., Madhuprakash, Jogi., Podile, Appa Rao., (2016) , A new chitinase-D from a plant growth promoting Serratia marcescens GPS5 for enzymatic conversion of chitin , Bioresource Technology, Elsevier,220,,200-207.
14 Das, Subha Narayan., Madhuprakash, Jogi., Sarma, PVSRN., Purushotham, Pallinti., Suma, Katta., Manjeet, Kaur., Rambabu, Samudrala., Gueddari, Nour Eddine El., Moerschbacher, Bruno M., Podile, Appa Rao., (2015) , Biotechnological approaches for field applications of chitooligosaccharides (COS) to induce innate immunity in plants , Critical Reviews in Biotechnology, Informa Healthcare,35,1,29-43.
13 Madhuprakash, Jogi., El Gueddari, Nour Eddine., Moerschbacher, Bruno M., Podile, Appa Rao., (2015) , Catalytic efficiency of chitinase-D on insoluble chitinous substrates was improved by fusing auxiliary domains , PLoS One, Public Library of Science,10,1,e0116823.
12 Madhuprakash, Jogi., El Gueddari, Nour Eddine., Moerschbacher, Bruno M., Podile, Appa Rao., (2015) , Production of bioactive chitosan oligosaccharides using the hypertransglycosylating chitinase-D from Serratia proteamaculans , Bioresource Technology, Elsevier,198,,503-509.
11 Madhuprakash, J., Bobbili, KB., Moerschbacher, BM., Singh, TP., Swamy, MJ., Podile, AR., (2015) , Inverse relationship between chitobiase and transglycosylation activities of chitinase-D from Serratia proteamaculans revealed by mutational and biophysical analyses. , Scientific Reports, Nature Group,5,,15657-15657.
10 Madhuprakash, Jogi., Tanneeru, Karunakar., Karlapudi, Bhavana., Guruprasad, Lalitha., Podile, Appa Rao., (2014) , Mutagenesis and molecular dynamics simulations revealed the chitooligosaccharide entry and exit points for chitinase D from Serratia proteamaculans , Biochimica et Biophysica Acta (BBA)-General Subjects, Elsevier,1840,9,2685-2694.
9 Madhuprakash, J., (2014) , Structural and mutational analysis of transglycosylation by chitinase D from Serratia proteamaculans , Thesis, Hyderabad,,,.
8 Madhuprakash, Jogi., Singh, Avinash., Kumar, Sanjit., Sinha, Mau., Kaur, Punit., Sharma, Sujata., Podile, Appa R., Singh, Tej P., (2013) , Structure of chitinase D from Serratia proteamaculans reveals the structural basis of its dual action of hydrolysis and transglycosylation , International Journal of Biochemistry and Molecular Biology, e-Century Publishing Corporation,4,4,166.
7 Sarma, Pullabhotla Venkata Subba Rama Narsimha., Prakash, Jogi Madhu., Das, Subha Narayan., Kaur, Manjeet., Purushotham, Pallinti., Podile, Appa Rao., (2013) , Microbial Chitinases: Natural Sources, Mutagenesis, and Directed Evolution to Obtain Thermophilic Counterparts , Thermophilic Microbes in Environmental and Industrial Biotechnology, Springer Netherlands,,,649-669.
6 AR Podile, SN Das, J Madhuprakash, PVSRN Sarma., (2013) , Transglycosylation mediated synthesis of chitooligosaccharides and scope of application. , Proceedings of the Indo-Mexico workshop on Biotechnology Beyond Borders., ,,,348-364.
5 Yadlapalli, Rama Krishna., Chourasia, OP., Jogi, Madhu Prakash., Podile, Appa Rao., Perali, Ramu Sridhar., (2013) , Design, synthesis and in vitro antimicrobial activity of novel phenylbenzamido-aminothiazole-based azasterol mimics , Medicinal Chemistry Research, Springer-Verlag,22,6,2975-2983.
4 Madhuprakash, Jogi., Tanneeru, Karunakar., Purushotham, Pallinti., Guruprasad, Lalitha., Podile, Appa Rao., (2012) , Transglycosylation by chitinase D from Serratia proteamaculans improved through altered substrate interactions , Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology,,,jbc. M112. 400879.
3 Das, SN., Madhuprakash, J., Podile, Appa Rao., (2012) , Elicitor active molecules from fungal cell wall , Molecular Approaches for Plant Fungal Disease Management, Westville Publishers,,,52-68.
2 Podile, Appa Rao., Purushotham, P., Das, SN., Sarma, PVSRN., Madhuprakash, Jogi., (2012) , Chitin, chitosan and chitooligosaccharides: A treasure in store , Microbial Diversity: Exploration and Bioprospecting, Scientific Publishers,,,309-324.
1 Das, SN., Neeraja, Ch., Sarma, PVSRN., Prakash, J Madhu., Purushotham, P., Kaur, Manjeet., Dutta, Swarnalee., Podile, AR., (2011) , Microbial chitinases for chitin waste management , Microorganisms in Environmental Management, Springer Netherlands,,,135-150.