Faculty Details

Faculty Details

  • Name: Prof. Dayananda Siddavattam
  • Mail: sdsluohyd.ernet.in
  • Contact: 040-23134578
  • Area Of Research:Molecular Microbiology, Environmental Microbiology, Regulation of Gene Expression

Administrative Experience

1. Head (Two terms), Dept. of Biochemistry, SK University, Anantapur.
2. Coordinator, Dept. of Biotechnology, SK University, Anantapur.
3. Head, Dept. of Animal Sciences (2007-2010), School of Life Sciences, University of Hyderabad, Hyderabad.
4. Coordinator (2012-2015), Research and Development, University of Hyderabad, Hyderabad.
5. Chief Proctor (2016-2019), University of Hyderabad.
6. Dean, School of Life Sciences (2019-till date), University of Hyderabad.


Awards and Honors

1. Fellow, National Academy of Sciences, Allahabad, India (NASI)
2. Fellow, Indian National Science Academy, New Delhi, India.
3. Fellow, Indian Academy of Sciences, Bangalore, India.
4. Andhra Pradesh Scientist Award-2008 – by The State Council of Science and Technology, Govt. of Andhra Pradesh, India.
5. Fellow, Andhra Pradesh Academy of Sciences (FAPAS), 2006.
6. Recipient of the International Research Development Award – by The Welcome Trust, UK.
7.  Recipient of UGC Mid-Career award-2019.

8. Recipient of JC Bose Fellowship Sponsored by Science and Engineering Research Board (SERB) – 2020.


Memberships of National Committees


1. Member, Projects Advisory Council (Fast Track), Dept. of Science and Technology, Ministry of Science and Technology, Govt. of India (2012-2015).

2. Member, Projects Advisory Council, BUILDER (Boost to University Interdisciplinary Life Science Departments Education and Research), Dept. of Biotechnology, Ministry of Science and Technology, Govt. of India. (2013-2016).

3. Member, Regulatory Council on Genetically Manipulated organisms (RCGM), Dept. of Biotechnology, Ministry of Science and Technology, Govt. of India (Since 2014-2016).

4. Member, Projects Advisory Council (Biochemistry, Biophysics, Molecular Biology & Microbiology), Dept. of Science and Technology, Ministry of Science and Technology, Govt. of India (2015-tilldate).

5. Member, DST-INSPIRE programme, Dept. of Science and Technology, Ministry of Science and Technology, Govt. of India (2015-tilldate).

6. Member, Sectional Committee, General Biology, Indian Academy of Sciences, Bangalore.

7. Member Sectional Committee, Microbiology and Immunology, Indian National Academy, New Delhi

8. Member, Review Committee, Scheme for Translational and Advanced Research in Science (STARS), Ministry of Human Resource Development, Govt. of India. 

9. Member, Task Force, Organismal and Evolutionary Biology, Science and Engineering Research Board (SERB), Dept. of Science and Technology, Govt. of India




Membership of Professional Bodies


1. Life Member, Society of Biological Chemists, India
2. Life Member, Indian Society for Comparative Animal Physiologists, India
3. Life Member, Indian Society for Cell Biologists, India
4. Life Member, Indian Society for Microbiologists, India

5. Secretary, Society of Biological Chemists (SBC), India (2014-2017).



Fellowships


1. UGC National Visiting Associate: (1985 to 1988 – three months in a year). Awarded by the University Grants Commission, New Delhi, to work in the laboratory of Prof. P. S. Sastry, Dept. of Biochemistry, Indian Institute of Science, Bangalore, India.
2. DAAD Fellow (1988-90): Awarded by the German Academic Exchange Service (DAAD), Ministry of Science and Technology, Federal Republic of Germany to work on plasmid encoded nif genes of Enterobacter agglomerans in the laboratory of Prof. Dr. W. Klingmuller, Institute for Genetics, University of Bayreuth, D-95440, Bayreuth, Germany.
 3. Visiting Scientist (1992-93): Sponsored by Ministry of Science and Technology (BMFT), Bonn, Germany, to work in the laboratory of Prof. Dr. W. Klingmuller.
 DAAD Fellow (short-term, May to July 1995): Awarded by the German Academic Exchange Service (DAAD), Germany, to continue the collaborative research activities with Prof. Dr. W. Klingmuller.
 4. Commonwealth Academic Staff Fellow (1995-1996): Sponsored jointly by the British Council, London, and the University Grants Commission, New Delhi to conduct postdoctoral research in collaboration with Dr. M. J. Merrick, Nitrogen Fixation Laboratory, John Innes Centre, Norwich NR4 7UH, U. K.
5. Visiting Scientist (2005-07-three months in each year): Sponsored jointly by National Sciences Foundation, USA and the Dept. of Science and Technology, New Delhi, to execute collaborative research work with Prof. J. Wild, Dept. of Biochemistry, Texas A&M University, Texas, USA (2007 to 2010, three months in a year).
6. DAAD Fellow ( September to November, 2010 ): Awarded by the German Academic Exchange Service (DAAD), Germany, to work as a Visiting Scientist in the Dept. of Microbiology, University of Halle, Germany.


Collaboration with Industry and Industry funded projects


1. Heterologous expression and large scale purification of recombinant proteins sponsored by Biocin Biotech Pvt Ltd., Hyderabad. (2005-2006). 10 lakhs.
2. Strain manipulations for enhanced production of biofuels from agriculture waste. Sponsored by Nagarjuna Chemicals and Fertilizers Ltd., Hyderabad. (2006-09), 21 lakhs.
3. Construction of T7 polymerase based expression vectors for expression of recombinant proteins in Pseudomonas florescence sponsored by Biological E Ltd, Hyderabad (2010- 11). 24 lakhs.
4. Heterologous expression of recombinant HPV coat protein, a vaccine candidate to prevent cervical cancer: Sponsored by Biological E Pvt Ltd., Hyderabad. 2011-2014), 24.00 lakhs.
5. Screening of microbial enzymes to develop green process in the pharmaceutical industry: Sponsored by M/s Aurabindo Pharma Pvt Ltd, Hyderabad. (2014-215), 20 lakhs.
6. Isolation and taxonomic characterization of drug-resistant bacteria from the effluent and soil samples collected from the bulk drug manufacturing units located in Hyderabad sponsored by The Bulk Drug Manufacturing Association of India, Hyderabad. (2016-2017), 20.2 lakhs.

7. Chemical and genomic approaches to investigate possible link between development of antimicrobial resistance (AMR) and pharma industry effluents sponsored by Bulk Drugs Manufacturers Association of India, Hyderabad. (2019-2021), 1.08 Crore.


Research Interests

Phosphotriesterases:

Bacterial Phosphotriesterases (PTEs) have gained prominence due to their involvement in the hydrolysis of triester linkage found in organophosphate (OP) insecticides and nerve agents. These PTEs have been classified into three distinct structural groups, viz organophosphate hydrolases (OPH), methyl parathion hydrolase (MPH) and Organophosphate Acid Anhydrolase (OPAA). Among these three PTEs, the OPAA is shown to be a dipeptidase (prolidase). However, quite independent views exist on the physiological role of the other two PTEs. One of them proposes an evolution of PTEs from their progenitors viz, lactonases (OPH) and lactamase (MPH), in response to increased concentration of organophosphate insecticide residues in soil. The second view suggests their role in iron/phosphate acquisition and believes that OPs are fortuitous substrates for PTEs. Although the proposed views are based on logic-driven science done in our own laboratory and elsewhere in the world, the conclusions drawn are quite different and suggests unique physiological roles for bacterial PTEs.


Horizontal Gene Transfer of opd island:

Research work done in our laboratory has shown for the first time organization of opd gene cluster as a mobile genetic element(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516476/pdf/1541.pdf). We have shown lateral mobility of opd cluster via Mobilizable/ self transmissible plasmids and also demonstrated its lateral transfer through transposition and site-specific integration(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516476/pdf/1541.pdf). Interestingly the opd island encoded esterase has induced metabolic diversion in E. coli and promoted its growth on less preferred carbon compounds
(http://www.jbc.org/content/early/2015/10/09/jbc.M115.661249.full.pdf). We have shown that this esterase induced metabolic diversion is due to sRNA mediated translational inhibition of mRNAs coding transcriptional factors FadR and HcaR. Our findings if seen together with the research work done elsewhere on the evolution of phosphotriesterases (PTEs), clearly supports evolution of PTEs in response to the accumulation of OP insecticide residues in the soil.


OPH exists as part of the multi-protein complex:

Our research on OPH biology revealed interesting observations in B. diminuta (reclassified as Sphingophyxis wildii) used as a model organism(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5381565/pdf/evw275.pdf). We have shown for the first time its dependency on Tat translocation machinery for membrane targeting. Its inner membrane topology and mechanism of membrane anchoring is fully elucidated by performing classical biochemical studies (http://jb.asm.org/content/191/20/6292.full.pdf+html,http://www.jbc.org/content/early/2016/02/09/jbc.M116.715110.full.pdf). These findings clearly suggested to revisit OPH biology. Tat substrates are membrane proteins requiring large cofactors or multiprotein complexes. OPH is clearly a metallo-enzyme and contains zinc as a cofactor. It is not clear why OPH take Tat route to target membrane? Our lab is currently working to find if OPH has any role, other than phosphotriesterases activity. Our unpublished research gained significant leads to suggest a physiological role of PTEs in soil bacteria.


Bacterial small RNAs:

The small RNAs play a critical role in post-transcriptional gene regulation. We have shown the opd island induced metabolic diversion is due to sRNA mediated translational inhibition of certain novel transcriptional factors that contribute for the induction of alternate carbon catabolic genes (http://www.jbc.org/content/early/2015/10/09/jbc.M115.661249.full.pdf).   Acinetobacter baumannii is a clinically significant organism. During 70s most of the A. baumannii strains were sensitive to known antibiotics. Today there are multidrug-resistant strains. Evolution of drug-resistant strains in less than five decades is puzzling and developing control strategies are scientifically challenging. We are focusing on identifying sRNAs that regulate carbon catabolic pathways in Acinetobacter baumannii. Our research is expected generate novel drug targets. We have also shown the existence of non-lytic phage in Acinetobacter baumannii. We are studying its mechanism of infection and working on its manipulation to use as a delivery system for peptide antibiotics.


Technology Transfer:

Organophosphate hydrolase, one of the PTEs we have been working on has shown broad substrate range and high catalytic efficiency. It has hydrolyzed certain OP insecticides and nerve agents at a rate close to substrate diffusion limits. We have therefore worked closely with laboratories of Defence Research and Development Establishment (DRDE), Gwalior and Central Scientific Instruments Organization (CSIO), Chandigarh to develop OPH centric technologies for detection and decontamination of OP insecticide and nerve gas agents. The expression and large scale purification systems developed in our laboratory were transferred to DRDE, Gwalior (pdf) for developing OPH based strategies for decontamination of OP nerve agents. The OPH based biosensors developed in collaboration with CSIO are capable of detecting parts per billion amounts of op residues.
(https://www.sciencedirect.com/science/article/pii/S0925400512000470).

No Records Available

Students/Projects Trainees

Name Joined As Mail Contact
Parapatla Hari Research Scholar harinr11@gmail.com ---
Y N S Harshita Research Scholar nagasai_harshita@yahoo.co.in ---
Aparna Nandavaram Research Scholar aparna.pcu@gmail.com ---
Annapoorni Lakshmansagar Research Scholar annapoornilakshmansagar@gmail.com ---
Ganeshwari Dhurve Research Scholar ganeshwaridhurve@gmail.com ---
Sandhya Behera Research Scholar sandhyabehera721@gmail.com ---
Jayaprakash Nayak Research Scholar jpnayakbdk96@gmail.com ---
Devyani Samantarrai Research Associate devyani66@gmail.com ---
Roja Rani Pallavali Senior Researcher p.rojarani.virology@gmail.com ---

Former Students/Projects Trainees

Name Joined As Working
B. Madan Mohan PhD Student Research Scientist, Ohio State University, USA
C. Suresh Kumar Research Scholar Professor, SKU, Anantapur
S. Sita Research Scholar Research Asst. Professor, Ohio State University, USA
B. Manavathi Research Scholar Professor, University of Hyderabad, Hyderabad
S. Khajamohiddin Research Scholar Professor, Uni. of Cape Town, South Africa
Suresh B. Pakala Research Scholar Associate Professor, IISER, Tirupati
R. Elisa Raju Research Scholar Staff Scientist, Invitrogen, Bangalore
G. Purushotham Research Scholar Post. Doc. Scientist, University of Texas, Tyler, USA
P. Aleem Basha Research Scholar Asst. Professor, Rayalaseema University, Kurnool
P. Emmanuel Vijay Paul Research Scholar Scientist, Aurabindo Pharma , Hyderabad
Toshi Sangbha Research Scholar Post. Doc. Scientist, IRRI, Philippines
Devi Prasanna Chekka Research Scholar Scientist, Biological E. Pvt Ltd. Hyd
Swetha Kamireddy Research Scholar Scientist/Manager, Biological E. Pvt Ltd., Hyderabad
Sunil Parthasarathy Research Scholar Scientist, Aurabindo Pharma , Hyderabad
Ashok K. Madikonda Research Scholar Post doctoral Scientist, Academia Sinica, Taiwan
Ramamurty Gudla Research Scholar Research Scientist, University of Hyderabad
65 Hari Parapatla, Ramurthy Gudla, Guruprasad Varma Konduru, Elsin Raju Devadasu,. Hampapathula Adimurthy Nagarajaram, Manjula Sritharan, Rajagopal Subramanyam and Dayananda Siddavattam (2020) , Organophosphate hydrolase interacts with ferric-enterobactin and promotes iron uptake in association with TonB dependent transport system , Biochemical Journal, ,477,,2821-2840 IF - 4.331.
64 Devyani Samantarrai, Yakkala Naga Sai Harshita and Dayananda Siddavattam (2020) , Analysis of indigenous plasmid sequences of A. baumannii DS002 reveal existence of lateral mobility and extensive genetic recombination among Acinetobacter plasmids. , J Genetics , ,,,.
63 Ashok Madikonda, Shaikh Akbarpasha, Sonali Khanra, Harshita Yakkala, Sailu Yellaboina, and Sue Lin-Chao and Siddavattam D (2020) , A prophage e14-encoded small RNA, co293, inhibits translation of transcription factors HcaR and FadR , FEBS J. , ,,,.
62 Devyani Samantarrai, Annapoorni Lakshman Sagar, Ramurthy Gudla and Dayananda Siddavattam (2020) , Ton-B dependent transporters in Sphingomonads :Unraveling their distribution and function in environmental adaptation , Microorganisms, ,,,.
61 Ramurthy Gudla1, Guruprasad Varma Konduru2, Hampapathalu Adimurty Nagarajaram3, and Dayananda Siddavattam (2019) , Organophosphate hydrolase interacts with Ton components and targets membrane only in presence of ExbB/ExbD complex , FEBS Letters, ,593,,581-593.
60 Azam Sarwar, Sunil Parthasarathy, Chhaya Singh and Dayananda Siddavattam (2019) , Genome organization and adaptive potential of archetypal organophosphate degrading Sphingobium fuliginis ATCC 27551 , Genome Biology Evolution, ,,,.
59 Mehta J, Dhaka S, Paul AK, Siddavattam D, Deep A (2019) , Organophosphate hydrolase conjugated UiO 66-NH2 MOF based highly sensitive optical detection of methyl parathion , Environ Res, ,,,.
58 Purohit HJ, Siddavattam D and Phale P (2019) , Microbe-Assisted Biodegradation, Bioremediation and Metabolic Engineering , Proc Indian Natn Sci Acad , ,,,.
57 Yakkala H, Samantarrai, D Michael Gribskov M and Siddavattam D (2019) , Comparative Genomics reveal niche-specific unique genome expansion in Acinetobacter baumannii strains , PLoS ONE, ,,,.
56 Gudla R, Konduru GV, Nagarajaram HA, Sritharan M, and Siddavattam D (2019) , Organophosphate hydrolase is a part of novel Ton complex in Sphingobium fuliginis , FEBS Letters, ,,,.
55 Jyotsana Mehta, Sarita Dhaka, Neha Bhardwaj, A K Paul, Siddavattam Dayananda, Sung-Eun Lee, Ki-Hyun Kim, Akash Deep (2019) , Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion. , Sensors and Actuators B. , ,,,.
54 Siddavattam D, Yekkala Y and Samantarrai D (2019) , Lateral Transfer of Organophosphate Degradation (opd) Genes among Soil Bacteria:Mode of Transfer and Contributions to Organismal Fitness. , J. Genetics, ,,,.
53 Parthasarathy S, Parapatla H, Siddavattam D (2017) , Topological analysis of the lipoprotein organophosphate hydrolase from Sphingopyxis wildii reveals a periplasmic localisation. , FEMS Microbiol Lett, ,,,.
52 Parthasarathy S, Gudla R and Siddavattam D (2017) , Evolution of Phosphotriesterases (PTEs): How Bacteria Can Acquire New Degradative Functions? , Proc Indian Natn Sci Acad , ,,,.
51 Parthasarathy S, Azam S, Lakshman Sagar A, Narasimha Rao V, Gudla R, Parapatla H, Yakkala H, Ghanta Vemuri S, Siddavattam D (2017) , Genome-Guided Insights Reveal Organophosphate-Degrading Brevundimonas diminuta as Sphingopyxis wildii and Define Its Versatile Metabolic Capabilities and Environmental Adaptations. , Genome Biol Evol, ,,,.
50 Sunil Parthasarathy, Hari Paripatla, Tracy Palmer and Siddavattam D (2016) , Organophosphate Hydrolase is a lipoprotein and interacts with Pi-specific transport system to facilitate growth of Brevudimonas diminuta using OP insecticide as source of phosphate. , J. Biol. Chem, ,,,.
49 Nandavaram A, Lakshman Sagar A, Madikonda AK, Siddavattam D. (2016) , Proteomics of Sphingobium indicum B90A for a deeper understanding of hexachlorocyclohexane (HCH) bioremediation. , Rev Environ Health, ,,,.
48 Deviprasanna Chakka, Ramurthy Gudla, Ashok Kumar Madikonda, Emmanuel Vijay Paul Pandeeti, Sunil Parthasarathy and Dayananda Siddavattam (2015) , The organophosphate degradation (opd) island borne esterase induced metabolic diversion in E. coli and its influence on p-nitrophenol degradation. , J Biol. Chem , ,,,.
47 Longkumer T, Parthasarathy S, Vemuri SG, Siddavattam D (2014) , OxyR-dependent expression of a novel glutathione S-transferase (Abgst01) gene in Acinetobacter baumannii DS002 and its role in biotransformation of organophosphate insecticides , Microbiology, ,,,.
46 Longkumer T, Kamireddy S, Muthyala VR, Akbarpasha S, Pitchika GK, Kodetham G, Ayaluru M, Siddavattam D (2013) , Acinetobacter phage genome is similar to Sphinx 2.36, the circular DNA copurified with TSE infected particles. , Sci Rep, ,,,.
45 Pandeeti EV, Longkumer T, Chakka D, Muthyala VR, Parthasarathy S, Madugundu AK, Ghanta S, Medipally SR, Pantula SC, Yekkala H, Siddavattam D (2013) , Multiple mechanisms contribute to lateral transfer of an organophosphate degradation (opd) island in Sphingobium fuliginis ATCC 27551. , G3: Genes Genomes and Genetics (Genetics Society of America: Bethesda)., ,,,.
44 Pinjari AB, Kamireddy S, Pandey JP and Siddavattam D (2013) , Expression and subcellular localization of Organophosphate Hydrolase (OPH) in acephate degrading Pseudomonas sp. strain Ind01 and its use in eliminating residues of organophosphate (OP) insecticides. , Let. Appl. Microbiol, ,,,.
43 Shaveena Thakura, Pardeep Kumarb, M. Venkateswar Reddyc, D. Siddavattamc, A.K. Paul (2013) , Enhancement in sensitivity of fluorescence based assay for organophosphatesdetection by silica coated silver nanoparticles using organophosphate hydrolase. , Sensors and Actuators B , ,,,.
42 Thakur S, Venkateswar Reddy M , Siddavattam D, Paul AK (2012) , A fluorescence based assay with pyranine labeled hexa-histidine tagged organophosphorus hydrolase (OPH) for determination of organophosphates , Sensors and Actuators B , ,,,.
41 Kumar SS, Kumar MS, Siddavattam D, Karegoudar TB (2012) , Generation of continuous packed bed reactor with PVA-alginate blend immobilized Ochrobactrum sp. DGVK1 cells for effective removal of N,N-dimethylformamide from industrial effluents. , J Hazard Mater, ,,,.
40 Pinjari AB, Novikov B, Yohannes H. Rezenom YH,Russell DH, Wales ME, and Siddavattam D (2012) , Pseudomonas jinjuensis initiates mineralization of acephate in environmental samples , PLoS One, ,,,.
39 Siddavattam D, Karegowdar TB, Mudde SKKumar N, Baddam R, Avasthi TS and Ahmed N (2011) , Genome of novel isolate of Paracoccus denitrificans capable of degrading N, N-dimethylformamide , J Bacteriol, ,,,.
38 Pandeeti EVP, Pitchika G, Jotshi J, Nilegaonkar SS, Kanekar PP and Siddavattam D (2011) , Enzymatic depilation of animal hide: Identification of elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a depilating protease , PLoS ONE , ,,,.
37 Pandeeti EVP, Chakka D, Pandey JP and Siddavattam D (2011) , Indigenous organophosphate-degrading (opd) plasmid pCMS1 of Brevundimonas diminuta is self-transmissible and plays a key role in horizontal mobility of the opd gene. , Plasmid, ,,,.
36 Parthasarathy S, Sastry PS and Siddavattam D (2011) , Transport of prefolded proteins in bacteria: An overview on Twin Arginine Transport (Tat) Pathway and its role in Pathogenesis. , Current Sci. , ,,,.
35 Pandeeti EVP and Siddavattam (2011) , Purification and characterization of catechol 1, 2 dioxygenase from Acinetobacter sp. DS002 and cloning, sequencing of partial catA gene. , Indian J Microbiol, ,,,.
34 Kanugula AKR, Repalle ER, Pandey JP, Sripad G, Mitra CK, Dubey DK and Siddavattam D (2010) , Immobilization of organophosphate hydrolase (OPH) on biocompatible gelatin pads and its use in removal of OP compounds and nerve agents. , Ind. J. Biochem. Biophys. , ,,,.
33 Pandeti EVP, Kamireddy S, Langekumer T, Parthasarathy S, Ashok Kumar and Siddavattam D (2010) , Bacterial Small RNAs (sRNAs) and Carbon Catabolite Repression. In Microbes in Environment and Sustainable Agriculture. , Ed. Tulasi S, Johri.MM, Springer International., ,,,.
32 Pinjari, AB, Parthasarathy S, Paul EVP, Chakka D, Langekumer T, Kamireddy S and Siddavattam D (2010) , Microbial metabolism of organophosphates: A journey from biodegradation to bioremediation. , Applied Microbiology and Microbial Biotechnology, ,,,.
31 Gorla P, Pandey JP, Parthasarathy S, Merrick M, Siddavattam D. (2009) , Organophosphate hydrolase in Brevundimonas diminuta is targeted to the periplasmic face of the inner membrane by the twin arginine translocation pathway. , J Bacteriol, ,,,.
30 Pandey JP, Gorla P, Manavathi B and Dayananda Siddavattam (2009) , mRNA secondary structure modulates the translation of organophosphate hydrolase (OPH) in E. coli. , Mol. Biol. Rep, ,,,.
29 Pandeeti EV, Chinnaboina MR, Siddavattam D (2009) , Benzoate-mediated changes on expression profile of soluble proteins in Serratia sp. DS001. , Lett Appl Microbiol., ,,,.
28 Khajamohiddin S, Repalle ER, Pinjari AB, Merrick M, Siddavattam D (2008) , Biodegradation of aromatic compounds: an overview of meta-fission product hydrolases. , Crit Rev Microbiol, ,,,.
27 Rasineni GK, Siddavattam D and Reddy AR (2008) , Free radical quenching activity and polyphenols in three species of Coleus , J Med. Plant Res. , ,,,.
26 Pakala SB, Gorla P, Pinjari AB, Krovidi RK, Baru R, Yanamandra M, Merrick M, Dayananda Siddavattam (2007) , Biodegradation of methyl parathion and p-nitrophenol: evidence for the presence of a p-nitrophenol 2-hydroxylase in a Gram-negative Serratia sp. strain DS001. , Appl. Microbiol. Biotechnol, ,,,.
25 Khajamohiddin S, Babu PS, Chakka D, Merrick M, Bhaduri A, Sowdhamini R, Dayananda Siddavattam . (2006) , A novel meta-cleavage product hydrolase from Flavobacterium sp. ATCC27551. , Biochem Biophys Res Commun, ,,,.
24 Dayananda Siddavattam, Elisa Raju Emmanuel V. Paul and Mike. Merrick (2006) , Overexpression of opd gene stimulates the synthesis of outer membrane porin OmpF in E. coli BL21. , Pest. Physiol. Biochem, ,,,.
23 Veerana Gouda, Paul EV, Purushotham Gorla, Dayananda Siddavattam and Karegoudar (2005) , Complete mineralisation of dimethylformamide by Ochrobactrum sp. DGVK1 isolated from the soil samples collected from the coalmine leftovers. , Appl. Microbiol. Biotechnol. , ,,,.
22 Manavathi B, Pakala SB, Purushotham G Merrick MJ and Siddavattam D (2005) , Regulation of plasmid encoded organophosphate (opd) degrading gene in Flavobacterium sp. , Pest. Biochem. Physiol., ,,,.
21 Kalari Satishkumar; Dayananda Siddavattam and Subramanyam Chivukula (2005) , Copper alone, but not oxidative stress, induces copper-metallothionein gene in Neurospora crassa , FEMS Microbiol. Lett. , ,,,.
20 Hussain Reddy K, Surendra Babu M, Suresh B. Pakala and Dayananda Siddavattam (2004) , Synthesis, Characterization and nuclease activity of copper (II), nickel (II), cobalt (II) and iron(II) complexes with oxime-thiosemicarbazones. , Ind. J. Chem. , ,,,.
19 Siddavattam D, Khaja Mohiddin S, Manavathi B, Suresh Babu P and Merrick MJ (2003) , Transposon-like organisation of the plasmid-borne organophosphate degradation (opd) gene cluster found in Flavobacterium sp. , Appl. Environ. Microbiol. , ,,,.
18 Somara S, Manavathi B, Tebbe CC and Siddavattam (2002) , Localization of identical organophosphorus pesticide degrading (opd) genes on genetically dissimilar indigenous plasmids of soil bacteria: PCR amplification, cloning and sequencing of the opd gene from Flavobacterium balustinum. , Ind. J. Exp. Biol. , ,,,.
17 Somara S, manavathi B, Tebbe CC and Siddavattam (2002) , Over-expression of parathion hydrolase of Flavobacterium balustinum as His-tagged protein in E. coli: Purification and characterization of His-tagged parathion hydrolase. , Ind. J. Biochem. Biophys. , ,,,.
16 Manavathi B, Pakala SB, Khaja Mohiddin S, Purushotham G and Siddavattam D (2002) , Genetic analysis of organophosphorus pesticide degradation (opd) in Flavobacterium sp. : organisation and regulation of expression of genes involved in degradation of op-pesticides. , In. proccedings of National symposium on developments in Microbial Biochemistry and its impact on Biotechnology, ,,,.
15 Manavati M, Somara S and Siddavattam D (1999) , Indigenous organophosphate pesticide degrading (opd) plasmid pBC9 of Flavobacterium balustinum: construction of plasmid library and detection of clone with opd gene. , Ind. J. Micro. Biol., ,,,.
14 Sita S and Siddavattam D (1995) , Plasmid mediated organophosphorus pesticide degradation by Flavobacterium balustinum. , Biochem Mol Biol Int , ,,,.
13 Siddavattam D, Hans-Dieter Steibl, Kreutzer R and Klingmüller W (1995) , Regulation of nif gene expression in Enterobacter agglomerans: Nucleotide sequence of nifLA operon and influence of temperature and ammonium on its transcription. , Mol Gen Genet , ,,,.
12 Siddavattam D, Nickles A, Herterich S, Steibl H-D, Kreutzer R and Klingmüller W (1995) , Site-specific mutagenesis in Enterobacter agglomerans: Construction of nifB mutants and analysis of the gene’s structure and function. , Mol Gen Genet , ,,,.
11 Steibl H-D, Siddavattam D and Klingmüller W (1995) , Molecular cloning of nif cluster and evidence for existence of similar nif clusters on dissimilar plasmids of Enterobacter strains. , Plasmid , ,,,.
10 Steibl H-D, Misch A, Steibl R, Siddavattam D, Deuerling E, Lechler A, Lewecke FM, Nickles A, Schwickerath O, Romanowski G and Klingmüller W (1994) , Nif plasmids of Enterobacter agglomerans strains. In: Kiss GB and Endre G (eds) Proceedings of the Ist European Nitrogen fixation conference , In: Kiss GB and Endre G (eds) Proceedings of the Ist European Nitrogen fixation conference. Officiana Press, Szeged, ,,,.
9 Siddavattam D, Singh M and Klingmüller W (1993) , Structure of nifQ gene from Enterobacter agglomerans and its over expression in Escherichia coli. , Mol Gen Genet, ,,,.
8 Steibl H-D, Siddavattam D and Klingmüller W (1992) , Similar nif cluster on dissimilar plasmids of Enterobacter strains. , In: Palacios R and Mora J (eds) New Horizons in Nitrogen Fixation. Kluwer Academic Publishers, Dordrecht, ,,,.
7 Steibl R, Steibl H-D, Siddavattam D and Klingmüller W (1992) , Identification of new nif gene nifI in the nif cluster of Enterobacter agglomerans 333. , In: Palacios R and Mora J (eds) New Horizons in Nitrogen Fixation . Kluwer Academic Publishers, Dordrecht, ,,,.
6 Kreutzer R, Siddavattam D and Klingmüller W (1991) , Cotranscription of the electrontransport genes nifJ and nifF in Enterobacter agglomerans , J Bacteriol , ,,,.
5 Kreutzer R, Steibl H-D, Siddavattam D, Dippe R, Halda L, Buck M and Klingmüller W (1991) , Genetic characterisation of nitrogen fixation in Enterobacter strains from the rhizosphere of cerials , In: Polsinelli M, Materassi R and Vinenzini M (eds) Nitrogen Fixation. Kluwer Academic Publishers, Dordrecht, Boston, London,, ,,,.
4 Siddavattam D, Steibl H-D and Klingmüller W (1990) , Cloning and sequencing of nifF from pEA9, a self transmissible nif plasmid of Enterobacter agglomerans , New trends in Genetics, Resch Verlag, Bonn , ,,,.
3 Siddavattam D and Nandakumar NV (1987) , Colorimetric enzymatic determination of methyl parathion and paraoxon. , Acta Chemica Turcica , ,,,.
2 Siddavattam D, Usharani A, Ramamurthi R and Nandakumar NV (1986) , Fenitrothion degradation in soil , Poll Res , ,,,.
1 Siddavattam D and Nandakumar NV (1985) , Colorimetric quantification of chicken egg albumin cholinesterase inhibition for estimation of fenitrothion and its oxygen anolog. , J Food Sci Tech , ,,,.
Patent No/Application No Patent Title Authors Applied Year Granting Year
WO/2009/113101 GENETICALLY TRANSFORMED MICROORGANISM FOR BIOMASS FERMENTATION DAYANANDA SIDDAVATTAM, DODDALA ANITHA CHOUDARY, KARTHIKEYAN VENKATANARAYANAN 2009 2009
Title Year Duration Amount(In Lakhs) PI/I
EXPRESSION, PURIFICATION AND IMMUNOLOGICAL CHARACTERIZATION OF THE NANOPARTICLE ENCAPSULATED RBD, HR1, HR2 DOMAINS OF THE S PROTEINS OF SARS - COV2 2020 1 23 Prof. S. Dayananda
DBT-Centre for Microbial Informatics (DBT-CMI) (approved by the Taskforce, DBT; awaiting financial sanction) 2020 5 200 Prof. H.A.Nagarajaram (Coordinator and PI), Prof. Appa Rao Podile (Co-PI), Prof. Dayananda Siddavattam (Co-PI), Prof. Ch Venkataramana (Co-PI), Dr. Pankaj Singh (Co-PI), Dr. Vivek (Co-PI), Dr. Manjari Kiran (Co-PI)
Chemical and genomic approaches to investigate possible link between development of antimicrobial resistance (AMR) and pharma industry effluents 2019 2 120 Prof. Dayananda Siddavatttam (PI), Prof. H.A.Nagarajaram (Co-PI), Prof. Ch Venkataramana (Co-PI)
Chemical and genomic approaches to investigate possible link between development of antimicrobial resistance (AMR) and pharma industry effluents. 2019 3 100 Dayananda Siddavattam
Membrane associated organophosphate (OPH) hydrolase complex in sphingobium fuliginis: determination of inter subunit interactions and elucidation of its role in acquisition of phosphate or iron. 2017 3 58.5 Dayananda Siddavattam
Comprehensive omics studies on a versatile Sphingomonad strain to establish theoretical basis for in situ bioremediation of recalcitrant environmental pollutants. 2017 2 57.9 Dayananda Siddavattam
Role of non-coding small RNAs (sRNA) in regulation of carbon catabolic pathways In E. coli 2017 3 27.6 Dayananda Siddavattam