Faculty

Faculty Details


Faculty Details

  • Name: Prof. Sharmistha Banerjee
  • Mail: sbsluohyd.ac.in
  • Contact: 914023134573
  • Area Of Research:Molecular pathogenesis and immunology of Mycobacterium tuberculosis (M.tb), Human Immunodeficiency Virus (HIV) and M.tb-HIV co-infection.

Brief Profile

 Sharmistha Banerjee received her Ph.D. in Biochemistry in 2005 from University of Hyderabad. She worked at Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India, and later continued her postdoctoral studies at the Laboratory of Transcriptional Biology on mechanisms of Rho-dependent termination in E.coli. She worked briefly at Molecular Cardiology Unit, Lerner Research Institute, The Cleveland Clinic, Cleveland, USA on role of integrins in prostate cancer metastasis.

Presently, as a Professor in the Department of Biochemistry, School of Life Sciences, she teaches Immunology and Enzymology along with various topics on Infectious diseases and host pathogen interactions.

 

Research Interests

 The Research focus of her laboratory is molecular pathogenesis and immunology of Mycobacterium tuberculosis(M.tb), Human Immunodeficiency Virus (HIV) and M.tb-HIV co-infection.

 

The laboratory is working on two human pathogens, Mycobacterium tuberculosis [tuberculosis (TB) causing bacteria] and Human Immunodeficiency Virus (HIV), the synergistic pandemic of which is a major health concern in India and worldwide. The laboratory, in the last few years, has added important baseline information on molecular mechanisms behind pathogenesis of HIV and mycobacteria, during both mono-and co-infection, identifying potential drug targets and biomarkers. Presently, the following themes are under investigation:

 

(a) Cellular and Molecular Mechanisms of Mycobacterial physiology: Deciphering adaptive metabolic and regulatory networks of Mycobacterium tuberculosis in response to infection and related stresses through proteomics and metabolomics; identification and validation of critical enzymes as potential targets and screening small molecule inhibitors against these targets for anti-mycobacterial activity.

 (b) Molecular insights into HIV Biology: Investigating perturbations in nuclear architecture during HIV-1 infection and studying the molecular basis of differential regulation of viral persistence and propagation in diverse cell types.

(c) Molecular Mechanismsof M.tb-HIV co-infection: System-wide studies to identify the concurrent perturbations in the proteomes of both host cell and intracellular mycobacteria during HIV co-infection and deciphering the underlying molecular mechanisms.

 

Honours and Awards:

•           Chancellor’s Award 2017, University of Hyderabad

•           National Women Bioscientist Award (Young Category) 2016, from Department of Biotechnology, Govt. of India

•           Prof BK Bachhawat International Travel Grant for Young Scientists 2018

•           Innovative Young Biotechnologist Award (IYBA) 2011, from the Department of Biotechnology, Govt. of India

•           BioAsia Innovation Award 2008

•           INSA Young Scientist Award 2005, from the Indian National Science Academy.

 

No Records Available

Students/Projects Trainees

Name Joined As Mail Contact
Dr. Krishnaveni Mohareer PDF krishnaveni.mohareer@gmail.com ---
Kumaraswami Chintala PhD scholar swamy.chintala9@gmail.com ---
Jaya Shankar PhD scholar shankar.medikonda@gmail.com ---
Kiran Jakkala PhD scholar jkiranau@gmail.com ---
Govinda Raju Vadankula PhD scholar govindraj.vadankula@gmail.com ---
Sriram Yendrapelly PhD Scholar sriramyendrapelly@gmail.com ---
Mahesh Gauraram Lab manager gouraram2@gmail.com ---
Dr. Saima Naz Scientist under DHR-WSS saimanaz169@gmail.com ---
Satrupa Sarkar PhD Scholar satarupasarkar658@gmail.com ---
Siranjeevi R PhD Scholar siranjeeviharan12@gmail.com ---
Zade Omkar Shriram PhD scholar omkarzade02@gmail.com ---
Nandini Wankar PhD scholar wankarnandini@gmail.com ---
Anushka Agarwal PhD scholar anukartik70@gmail.com ---
Warisha Faiz Project Student warishafaiz.fz@gmail.com ---

Former Students/Projects Trainees

Name Joined As Working
Kannan BalaKrishnan PhD Scholar Postdoctoral Fellow, Cleveland Clinic, Florida, USA
Manasa N Project student Research Fellow, University of Würzburg, Germany
Geetika Arekuti Technical assistant Clinical Project Coordinator, Imaging Endpoints, Imaging research iCRO (till 2021)
Ronald Benzamin PhD scholar Assistant Researcher, University of California, San Francisco, USA
Atoshi Banerjee PhD scholar Research Analyst, University of Nevada, Las Vegas, USA
Rakesh Ganji PhD scholar Postdoctoral Scholar, Graduate School of Biomedical Sciences, Tufts University, Boston, USA
Harika Vemula Mani PhD scholar Research Associate, Cell Death
Iqra Bashir Nehvi PhD scholar
Suman Asalla PhD scholar Post-Doctoral Researcher, Heritage College of Osteopathic Medicine, Ohio University, Athens, USA
Arshad Rizvi PhD scholar Post-Doctoral Researcher, Emory University School of Medicine, Dept. of Microbiology and Immunology, Atlanta, USA
Harish Kumar Dubey Project JRF Technical Assistant, UoH-NIAB BSL3 facility, UoH, Hyderabad, India
Swetha Sankati Project assistant Executive Assistance, under Institution of Eminence, UoH, Hyderadad, India
Lavanya Priyadarshini Project JRF Entrepreneur, Hyderabad, India
Aakrati Bhat MSc project trainee Project assistant, CCMB, Hyderabad, India
Archismitha Chaterjee MSc project trainee PhD Scholar at NBRC, India
Charul Goel MSc project trainee
Ananya Dash Int. MSc (Systems Biology) Project Student PhD scholar, Emory University, USA
56 Yandrapally S, Agarwal A, Chatterjee A, Sarkar S, Mohareer K, Banerjee S. (2023) , Mycobacterium tuberculosis EspR modulates Th1-Th2 shift by transcriptionally regulating IL-4, steering increased mycobacterial persistence and HIV propagation during co-infection. , Frontiers in Immunology, ,14,,.
55 Yandrapally S, Sarkar S, Banerjee S (2023) , HIV-1 Tat commandeers nuclear export of Rev-viral RNA complex by controlling hnRNPA2-mediated splicing. , Journal of Virology, ,,,doi: 10.1128/jvi.01044-23.
54 Conde R, Laires R, Gonçalves, LG, Rizvi A, Barroso C, Villar M, Macedo R, Simões MJ, Gaddam S, Lamosa P, Puchades-Carrasco L, Pineda-Lucena A, Patel AB, Mande SC, Banerjee S, Matzapetakis M, Coelho AV (2022) , Discovery of serum biomarkers for diagnosis of tuberculosis by NMR metabolomics including cross-validation with a second cohort. , Biomedical Journal, ,45,doi: 10.1016/j.bj.2021.07.006.,654-664.
53 Mohareer K and Banerjee S* (2022) , Zmp1 peptidase ({Mycobacterium}-type) , Handbook of Proteolytic Enzymes, 4e: Metallopeptidases, Edited by Neil Rawlings, Elsevier,1,4,.
52 Kanaparthi KJ, Afroz S, Minhas G, Moitra A, Khan RA, Medikonda J, Naz S, Cholleti NS, Banerjee S, Khan N (2022) , Immunogenic profiling of Mycobacterium tuberculosis DosR protein Rv0569 reveals its ability to switch on Th1 based immunity. , Immunol Lett, Elsevier,S0165-2478(22)00001-3,,.
51 Balakrishnan K, Munusami P, Mohareer K, Priyakumar UD, Banerjee A, Luedde T, Mande SC, Münk C*, Banerjee S* (2022) , Staufen-2 functions as a cofactor for enhanced Rev-mediated nucleocytoplasmic trafficking of HIV-1 genomic RNA via the CRM1 pathway. , The FEBS Journal, FEBS PRESS,289,,6731-6751.
50 Sarkar S, Balakrishnan K, Chintala K, Mohareer K, Luedde T, Jaguva Vasudevan AA, Münk C and Banerjee S* (2022) , Tough Way In, Tough Way Out: The Complex Interplay of Host and Viral Factors in Nucleocytoplasmic Trafficking during HIV-1 Infection. , Viruses, MDPI,14,,2503.
49 Yandrapally S, Mohareer K, Arekuti G, Vadankula GR, and Banerjee S (2021) , HIV co-receptor-tropism: cellular and molecular events behind the enigmatic co-receptor switching. , Critical Reviews in Microbiology, ,47,,499-516.
48 Chintala K, Mohareer K, Banerjee S (2021) , Dodging the host Interferon-Stimulated Gene mediated innate immunity by HIV-1: a brief update on intrinsic mechanisms and counter-mechanisms. , Frontiers in Immunology, ,12,,716927.
47 Balakrishnan K, Vasudevan AAJ, Mohareer K, Luedde T, Münk C*, Banerjee S* (2021) , Encapsidation of Staufen-2 Enhances Infectivity of HIV-1 , Viruses, MDPI,13, doi: 10.3390/v13122459,2459.
46 Nehvi IB, Quadir N, Khubaib M, Sheikh JA, Shariq M, Mohareer K, Banerjee S, Rahman SA, Ehtesham NZ, Hasnain SE (2021) , ArgD of Mycobacterium tuberculosis is a functional N-acetylornithine aminotransferase with moonlighting function as an effective immune modulator. doi: 10.1016/j.ijmm.2021.151544 , Int J Med Microbiol. , Elsevier,312,1,151544.
45 Albors-Vaquer A (1), Rizvi A (1), Matzapetakis M, Lamosa P, Coelho AV, Patel AB, Mande SC, Gaddam S, Pineda-Lucena A, Bnerjee S* and Puchades-Carrasco L* (2020) , Active and prospective latent tuberculosis are associated with different metabolomic profiles: Clinical potential for the identification of rapid and non-invasive biomarkers. , Emerg Microbes Infect , ,9,,1131-1139.
44 Mohareer K*, Medikonda S, V Govinda Raju, Banerjee S* (2020) , Mycobacterial control of host mitochondria: bioenergetic and metabolic changes shaping cell fate and infection outcome , Front Cell and Infect Microbiol, ,,,.
43 1. Vasudevan AAJ1, Balakrishnan K1, Gertzen CGW, Borvető F, Zhang Z, Sangwiman A, Held U, Küstermann C, Banerjee S, Schumann GG, Häussinger D, Bravo IG, Gohlke H, Münk C. (1 contributed equally) (2020) , Loop 1 of APOBEC3C regulates its antiviral activity against HIV-1. , J. Molecular Biology, ,,,.
42 Vasudevan AAJ(1), Balakrishnan K(1), Gertzen CGW, Borvető F, Zhang Z, Sangwiman A, Held U, Küstermann C, Banerjee S, Schumann GG, Häussinger D, Bravo IG, Gohlke H, Münk C. (2020) , Loop 1 of APOBEC3C regulates its antiviral activity against HIV-1. , J Mol Biol. doi: 10.1016/j.jmb.2020.10.014., ,,,.
41 Rizvi A, Shankar A, Chatterjee A, More TH, Bose T, Dutta A, Balakrishnan K, Madugulla L, Rapole S, Mande SS*, Banerjee S*, Mande SC*. (2019) , Rewiring of Metabolic Network in Mycobacterium tuberculosis During Adaptation to Different Stresses. , Front Microbiol. , Frontiers,10,2417,.
40 Rizvi A, Yousf S, Balakrishnan K, Dubey HK, Mande SC, Chugh J, Banerjee S . (2019) , Metabolomics studies to decipher stress responses in Mycobacterium smegmatis point to a putative pathway of methylated amines biosynthesis. , Jbact., ASM,201,15,707-718.
39 Kumar A, Phulera S, Rizvi A, Sonawane PJ, Panwar HS, Banerjee S, Sahu A, Mande SC. (2019) , Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis. , FEBS Letters, Wiley,593,,982-995.
38 Mohareer K, Asalla S, Banerjee S (2018) , Cell death at the cross roads of host-pathogen interaction in Mycobacterium tuberculosis infection , Tuberculosis (Edinb), Elsevier,113,,99-121.
37 Khadkikar P, Goud NS, Mohammed A, Ramamoorthy G, Ananthathatmula R, Doble M, Rizvi A, Banerjee S, Ravi A, Alvala (2018) , An efficient and facile green synthesis of bisindole methanes as potential Mtb FtsZ inhibitors. , Chem Biol Drug Des. , Wiley,92,,1933-1939.
36 Divya MB, Vemula M, Balakrishnan K, Banerjee S, Guruprasad L. (2018) , Mycobacterium tuberculosis PE1 and PE2 proteins carrying conserved a/b-serine hydrolase domain are esterases hydrolyzing short to medium chain p-nitrophenyl esters. , Prog Biophys Mol Biol., ,,,.
35 Asalla S, Mohareer K, Banerjee S. (2017) , Small molecule mediated restoration of mitochondrial function augments anti-mycobacterial activity of human macrophages subjected to cholesterol induced asymptomatic dyslipidemia. , Front. Cell. Infect. Microbiol. , Frontiers,,,.
34 Balakrishnan K, Mohareer K, Banerjee S. (2017) , Mycobacterium tuberculosis Rv1474c is a TetR-like transcriptional repressor that regulates aconitase, an essential enzyme and RNA-binding protein, in an iron-responsive manner. , Tuberculosis (Edinb.), Elsevier,103,,71–82.
33 Dev RR, Ganji R, Singh SP, Mahalingam S, Banerjee S, Khosla S. (2017) , Cytosine methylation by DNMT2 facilitates stability and survival of HIV-1 RNA in the host cell during infection , Biochem J, Portland Press,,,.
32 Reddy ER, Yaseen AM, Rizvi A, Deora GS, Banerjee S, Sevilimedu A, Rajadurai M. (2017) , Antibacterial Nanoparticles Based on Fluorescent 3-Substituted Uridine Analogue , Chemistry Select , Wiley,2,,557-561.
31 Vemula MH, Medisetti R, Ganji R, Jakkala K, Sankati S, Chatti K, Banerjee S. (2016) , Mycobacterium tuberculosis Zinc Metalloprotease-1 assists mycobacterial dissemination in Zebrafish. , Front Microbiol. , Frontiers,,,.
30 Vemula MH, Ganji R, Sivangala R, Jakkala K, Gaddam S, Penmetsa S, Banerjee S. (2016) , Mycobacterium tuberculosis Zinc Metalloprotease-1 Elicits Tuberculosis-Specific Humoral Immune Response Independent of Mycobacterial Load in Pulmonary and Extra-Pulmonary Tuberculosis Patients , Front Microbiol. , Frontiers,,,.
29 Ganji R, Dhali S, Rizvi A, Rapole S, Banerjee S. (2016) , Understanding HIV-Mycobacteria synergism through comparative proteomics of intra-phagosomal mycobacteria during mono- and HIV co-infection. , Scientific Reports , Nature,,,.
28 Sharma G, Sowpati DT, Singh P, Khan MZ, Ganji R, Upadhyay S, Banerjee S, Nandicoori VK, Khosla S. (2016) , Genome-wide non-CpG methylation of the host genome during M. tuberculosis infection , Scientific reports, Nature,,,.
27 Ganji R, Dhali S, Rizvi A, Sankati S, Vemula MH, Mahajan G, Rapole S, Banerjee S. (2016) , Proteomics approach to understand reduced clearance of mycobacteria and high viral titers during HIV-mycobacteria co-infection , Cellular Microbiology, Wiley,18,,355-368..
26 Sharma A, Rustag T, Mahajan G, Kumar A, Rao Kanury V. S, Banerjee S, Sherman DR and Mande SC (2016) , Towards understanding the biological function of the unusual chaperonin GroEL1 of Mycobacterium tuberculosis. , Tuberculosis (Edinb). , Elsevier,97,,137-146.
25 Ramaprasad EVV, Rizvi A, Banerjee S, Sasikala Ch, Ramana Ch.V. (2016) , Mycobacterium oryzae sp. nov., a scotochromogenic, rapidly growing species is able to infect human macrophage cell line. , Int J Syst Evol Microbiol, Microbiology Society,66,,4530-4536.
24 Banerjee A, Benjamin R, Balakrishnan K, Ghosh P and Banerjee S (2014) , Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev. Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev. , Retrovirology, Springer,11,,18.
23 Benjamin R, Banerjee A, Balakrishnan K, Sivangala R, Gaddam S, Banerjee S. (2014) , Mycobacterial and HIV Infections Up-Regulated Human Zinc Finger Protein 134, a Novel Positive Regulator of HIV-1 LTR Activity and Viral Propagation. , Plos One, Public Library of Science,9,8,e104908.
22 Suragani M, Aadinarayana VD, Pinjari AB, Tanneeru K, Guruprasad L, Banerjee S, Pandey S, Chaudhuri TK, Ehtesham NZ (2013) , Human resistin, a proinflammatory cytokine, shows chaperone-like activity. , Proc. Natl. Acad. Sci. USA. , National Academy of Sciences,110,51, 20467–20472.
21 Banerjee A, Benjamin R, Banerjee S. (2013) , Impact of viral factors on subcellular distribution and RNA export activity of HIV-1 rev in astrocytes 1321N1 , PLoS One, Public library of Science,,,.
20 Sultana R, Vemula MH, Banerjee S, Guruprasad L. (2013) , The PE16 (Rv1430) of Mycobacterium tuberculosis Is an Esterase Belonging to Serine Hydrolase Superfamily of Proteins. , PLoS ONE , Public library of Science,,,.
19 Mohareer K, Banerjee S and Hasnain SE. (2013) , Recombinant DNA technology in Eukaryotic gene expression system. , Textbook of Biochemistry, Biotechnology, Allied , Prentice Hall of India,,,.
18 Benjamin R, Banerjee A, Sunder SR, Gaddam S, Valluri VL and Banerjee S. (2013) , Discordance in CD4 T-Cell Levels and Viral Loads with Co-Occurrence of Elevated Peripheral TNF-a and IL-4 in Newly Diagnosed HIV-TB Co-Infected Cases. , PLoS One, ,,,.
17 Ramachary DB, Reddy YV, Atoshi Banerjee A and Banerjee S. (2011) , Design, synthesis and biological evaluation of optically pure functionalized spiro[5,5]undecane-1,5,9-triones as HIV-1 inhibitors. , Org. Biomol. Chem. , ,9,,7282-7286 .
16 Banerjee S, Farhana A, Ehtesham NZ, Hasnain SE. (2011) , Iron acquisition, assimilation and regulation in mycobacteria. , Infection, Genetics and Evolution , ,11,,825–838.
15 Hasnain SE, Banerjee S, Ehtesham NZ . (2011) , Translating advances in genomic research into clinical practice: The challenges ahead. , Med Princ. Pract., ,20,,392-394.
14 Banerjee S, Mohareer K, Hasnain SE. (2010) , Tuberculosis Related Immune Complications: A New Dilemma in Disease Management; in Biotechnology for sustainable development: achievements and challenges. , Biotechnology for sustainable development: achievements and challenges., ,,,143-151.
13 Banerjee S, Mohareer K, Hasnain SE. (2010) , Molecular diagnostics of MDR and XDR TB. , Ranbaxy Science Foundations, ,,,73-87.
12 Banerjee S, Siddiqi N, Hasnain SE . (2009) , Drug resistance tuberculosis. , Tuberculosis, Edited by Sharma SK and Alladi Mohan,,,.
11 Banerjee S, Nandyala A K, Podili R, Ahmed N and Hasnain SE. (2007) , Iron dependent RNA-binding activity of Mycobacterium tuberculosis aconitase. , J Bacteriol, ,189,,4046-4052..
10 Chalissery J, Banerjee S, Bandey I and Sen R . (2007) , Transcription termination defective mutants of Rho: role of different functions of Rho in releasing RNA from the elongation complex. , . J Mol Biol. , ,371,,855-872.
9 Pani B, Banerjee S, Chalissery J, Abhishek M, Malarini L R, Suganthan R, Sen R . (2006) , Mechanism of inhibition of Rho-dependent transcription termination by bacteriophage P4 protein PSU. , J Biol Chem , ,281,36,26491-500.
8 Banerjee S, Chalissery J, Bandey I and Sen R. (2006) , Rho-dependent transcription termination: more questions than answers. , J Microbiol. , ,44,,11-22.
7 Banerjee S, Nandyala A, Podili R, Katoch VM and Hasnain SE. (2005) , Comparison of Mycobacterium tuberculosis isocitrate dehydrogenases (ICD-1 and ICD-2) reveals differences in coenzyme affinity, oligomeric state, pH tolerance and phylogenetic affiliation. , BMC Biochemistry, ,29,,6-20.
6 Sah NK, Singh SN, Sahdev S, Banerjee S, Jha V, Khan Z, Hasnain SE. (2005) , Indian herb ‘Sanjeevani’ (Selaginella bryopteris) can promote growth and protect against heat shock and apoptotic activities of ultra violet and oxidative stress. , J Biosci., ,30,,499-505.
5 Banerjee S, Nandyala A, Podili R, Katoch VM, Murthy KJR and Hasnain SE. (2004) , Mycobacterium tuberculosis (M. tb) isocitrate dehydrogenases show strong B cell response and distinguish vaccinated controls from TB patients. , Proc. Natl. Acad. Sci. USA. , ,101,,12652- 12657. .
4 Siddiqi N, Das R, Pathak N, Banerjee S, Ahmed N, Katoch VM and Hasnain SE (2004) , Mycobacterium tuberculosis isolate with a distinct genomic identity overexpresses a TAP like efflux pump. , Infection. , ,32,,93-95 .
3 Ghosh S, Rasheedi S, Rahim SS, Banerjee S, Choudhary RK, Chakhaiyar P, Ehtesham NZ, Mukhopadhyay S, Hasnain, SE. (2004) , A novel method for enhancing solubility of the expressed proteins in E. coli. , Biotechniques, ,37,,418-424.
2 Siddiqi N, Shamim M, Hussain S, Choudhary RK, Ahmed N, Prachee, Banerjee S, Savithri GR, Alam M, Pathak N, Amin A, Hanief M, Katoch VM, Sharma SK and Hasnain SE. (2002) , Molecular Characterization of Multi-Drug Resistant Isolates of Mycobacterium tuberculosis From Patients in North-India. , Antimicrob. Agents Chemother., ,46,,443-450.
1 Ahmed N, Bal A, Khan AA, Alam M, Kagal A, Arjunwadkar V, Rajput A, Majeed AA, Rahman SA, Banerjee S, Joshi S, Bharadwaj R. (2002) , Whole genome fingperprinting and genotyping of multiple drug resistant (MDR) isolates of Pseudomonas aeruginosa from endophthalmitis patients in India. , Infection, Genetics and Evolution. , ,1,,1-6.
Title Year Duration Amount(In Lakhs) PI/I
Mitochondria-mediated metabolic crosstalk between host immune cells and Mycobacterium tuberculosis metabolic mutants during M1/M2 transition: a case study to understand the metabolic interlinking in the evolution of pathogenesis (SPR/2021/000137) 2022 3 63.79 Prof. Sharmistha Banerjee (PI); Prof. Nagarajaram (Co-I)
Proof of Principle and early safety testing in animal models of a novel lead compound against multi-drug resistant Mycobacterium tuberculosis strains 2021 3 450000000000 PI
Identification and Characterization of mycobacterial factors supporting HIV propagation during HIV-mycobacteria co-infection (BT/HRD/NWBA/38/09/2018) 2018 5 years 250000000000 Prof. Sharmistha Banerjee
Centre of Excellence in Biomolecular structure and function on Host-Pathogen interaction (BT/PR15450/COE/34/46/2016) 2016 5 years 761100000000 Prof. Sharmistha Banerjee

Title of Project

Date of Sanction and completion

Funding Agency

Grant ID

Amount (In Lakhs)

Status

Principal objective of the project

Mitochondria-mediated metabolic crosstalk between host immune cells and Mycobacterium tuberculosis metabolic mutants during M1/M2 transition: a case study to understand the metabolic interlinking in the evolution of pathogenesis

22 March, 2022 to 21st March 2025

SERB

SPR/2021/000137

63.79

Ongoing

Mapping of metabolic rewiring for stress adaptation by intracellular mycobacteria vis-à-vis changes in host metabolism

Proof of Principle and early safety testing in animal models of a novel lead compound against multi-drug resistant Mycobacterium tuberculosis strains

06-12-21 to 05-12-24

UoH-IoE RC4 grant

UoH-IoE-RC4-21-012

45.00

Ongoing

Validation of an in-house synthetic molecule as a lead molecule for antibacterial activities against multi-drug resistance tuberculosis.

Identification and Characterization of mycobacterial factors supporting HIV propagations during HIV- mycobacteria co-infection

 

18.07.2018

to 17.07.23

DBT, Govt. of India

(BT/HRD/NWBA/38/09/2018)”

25.00 lakhs

Ongoing

The project aims to study and characterize the Esx-3 secretome of opportunistic mycobacteria either alone or in tandem with HIV co-infection, to identify the mycobacterial factors that make the macrophage environment more conducive for HIV propagation, making these factors attractive for targeting to regulate the synergistic impact of mycobacteria on HIV.

 

Centre of Excellence in Biomolecular structure and function on Host-Pathogen interaction

28.12.16 to 27.06.22

DBT, Govt. of India

(BT/PR15450/COE/34/46/2016)

76.11 lakhs

Ongoing

 

Objectives include:

Characterization of Staufen-2 and Rev HIV  complex

To study the changes in the nuclear membrane architecture of HIV-1 permissive and HIV-1 restricting cells during the infection.

Mapping interactions of NPC components with HIV-1 components.

Functional  validation  of  interactions  and  their  role  in  HIV  infection  using  relevant model system such as yeast or cell lines based.

Assessment of antimicrobial and plant growth promoting potential of indigenous endophytic bacterial strains of Manipur”

01.09.2018

To 10.03.2022

DBT, Govt.

of India

(No.

BT/PR25395/NER/95/1177/2017)

 

15.36992

lakhs

Completed

Objectives includes characterization of active fraction of the new actinomycetes strain in the lab (SbAr) with anti-mycobacterial activity. Bulk purification of metabolite extracts from SBAr; HPLC fractionation of extracts;

Screening more actinomycete/bacterial strains for anti-mycobacterial activity. Bioactivity of various HPLC fractions, in isolation and in combination. Selection of

most active pure fraction; Screening more actinomycete/bacterial strains for anti-

mycobacterial activity. SBAr strain characterization; Screening more actinomycete/bacterial strains for anti-

mycobacterial activity (Contd.) and shortlisting a few on the basis of activity. Identification and characterization of the molecule from active pure fraction by LCMS, IR and NMR.

‘Design and Development of novel antitubercular agents targeting Shikimate kinase protein” under DBT.

 

09.01.2018

to 08.01.2021

DBT, Govt. of India

BT/PR23619/MED/29/1199/2017,

dated 09.01.2018)

21.6 lakhs

Completed

From 75 molecules screened from 5 series, we have now shortlisted some molecules to test on multi-drug resistant M. tuberculosis along with in-vitro cell-culture based infection assays

Identification and functional characterization of proteins from Mycobacterium tuberculosis targeting host-cell mitochondria

and studying their role in pathogenesis

25.05.16 to 24.05.19

DST

(EMR/2015/001029)

46.56 lakhs

Completed

We established a mechanistic link between dysfunctional mitochondria in macrophages exposed to sub-pathological levels of cholesterol for a chronic period as a potential risk factor for TB persistence. We observed that mitochondrial morphology and function were perturbed, more so during infection in cholesterol treated cells or in peripheral blood monocytes from clinically healthy individuals with borderline risk cholesterol levels. We also showed that pharmacological supplementation with small molecule M1 that restored mitochondrial structural and functional integrity restricted M.tb more effectively in cholesterol exposed macrophages.

Understanding the role of Rev- Staufen interactions in regulating HIV-1 propagation by altering cytoplasmic mRNA-silencing foci and nuclear membrane architecture

18.07.16 to 17.07.19

DBT

(BT/PR12626/MED/29/915/2014)

50.105

lakhs

Completed

We stablished that the Encapsidation of Staufen-2 Enhances Infectivity of HIV-1 and biochemicaly characterized the interaction identifying important residues. We found that Staufen-2 is incorporated into HIV-1, enhancing viral infectivity

SysTB: A network program for resolving the intracellular dynamics of host pathogen interaction in TB

infection

09.01.12 till 08.01.17

DBT, Govt. of India

(BT/PR3260/BRB/10/967/2011) PI

80.79 lakhs

Completed

Identification physiological processes supporting the survival of mycobacteria during co-infection and identified potential mycobacterial factors supporting survival of mycobacteria during mono- and  HIV co-infection

TBomics: An omics approach for diagnosing tuberculosis (New- Indigo-II)(BT/IN New Indigo/05/SB/2013) PI

02.07.13 to 31.12.16

DBT, Govt. of India International Collaboratio

n

 

55.95 lakhs

Completed

Identified a set of differential metabolites from the TB patient sera as potential biomarkers and identified that Mycobacterium tuberculosis Zinc Metalloprotease-1 Elicits Tuberculosis-Specific Humoral Immune Response Independent of Mycobacterial Load in Pulmonary and Extra-Pulmonary Tuberculosis Patients

Identification and chracterization of new iron-dependent post- transcriptional regulome of Mycobacteria

28.03.12 to 27.11.2017

DBT, Govt. of India

(BT/05/IYBA/2011)

54.18 lakhs

Completed

Identified and biochemically characterized a protein as transcription factor regulation expression of Mtb Aconitase in an iron-dependent manner

DBT Twin: Discovery of the bioactive natural products from microbes especially Actinimycetes in niche biotopes in Manipur

15.03.11 to 14.03.14

DBT, Govt. of India

(BT/47/NE/TBP/2010)

15.30 lakhs

Completed

Identified a novel strain of Streptomycetes which release anti-mycobacterial metabolite. The strain has been deposited for the patent purpose at Microbial Culture Collection – NCMR, Pune

DBT-Centre of Excellence- Identification of new therapeutic targets and immune activation markers in M. tb co-infections using comparative proteomics

30.12.08 to 30.06.14

DBT, Govt. of India

BT/01/COE/07/02 PI

61.25 lakhs

Completed

Established the cell culture-based co-infection model and found the differential expression of host proteins under mono- and co-infections

Physiological role of Rev in the cytoplasm of infected host cells: interactions with cytoplasmic host factors and association with viral pre-integration complex (PIC)

 

01.06.09 to 30.11.12

DBT, Govt. of India

BT/PR10092/BRB/10/585/2007

(letter II)

21.80 lakhs

Completed

Established that HIV-Rev localizes differentially in permissive and non-permissive cell lines and discovered a new factor Staufen2 which interacts with HIV-1 Rev and augments Rev activity and viral titers