An international expert on lipoxygenase research, Charité - Universitätsmedizin Berlin, Institute of Biochemistry, Germany. He works in the area of lipid signaling in health and disease. He explores the expression and regulation of pro-oxidative (lipoxygenases) and anti-oxidative (glutathione peroxidases) enzymes and on their characterization. At the molecular level his research involves experiments characterizing the structure-activity relation of lipoxygenases, glutathione peroxidases and DNA/RNA-binding proteins. This strategy involves targeted protein modification (side directed mutagenesis) and characterization of the induced functional alterations. At the cellular level he explores the functional relevance of the above-mentioned proteins for the cellular phenotype (cell physiology), which includes experiments on gene-modified cells (gain-of-function as well as loss-of-function strategies) and inhibitor studies. To reach this goal he employs transgenic mice that overexpress, knockout or knock in strategies for genes of interest.
Harvard Medical School and also the Director of the LC-MS Core Facility at Brigham and Women’s Hospital (BWH). He worked extensively on the purification of lipid molecules and in this process designed new chemical solvent systems which led to the isolation and discovery of a novel lipid, Mannosyl-Glucuronosyl-Diacylglycerol from Corynebacterium glutamicum (Cg). He also designed fractionation procedures to identify and purify two previously-indistinguishable variants of Cg lipopolysaccharide. At BWH he employed 2-D analytical and preparative TLC as well as intricate LC-MS fractionations for separation and characterisation of lipids and polysaccharides from biological sources ranging from microbes to mammals.
Dr. R B N Prasad, Indian Institute of Chemical Technology, Hyderabad