Course Details

    Duration: Ten Days: 5th December to 15th December, 2016

    Module A: Chemistry, biochemistry and pharmacology of lipid mediators

    Lecture 1 (90 min + 15 min of discussion)
    Introduction to lipid signaling and its biomedical impact. Lipid hormons beyond steroids and their roles for the homeostasis of living systems.
    Lecture 2 (60 min + 15 min of discussion) Prostaglandin as versatile signaling lipid hormones: Biosynthesis, degradation and biological implication
    Lecture 3 (60 min + 15 min discussion) Leukotrienes as mediators of inflammatory and allergic disorders: Biosynthesis, degradation of biological implications
    Lecture 4 (60 min + 15 min discussion) Anti-inflammatory eicosanoids (lipoxins, resolvins, protectins, maresins,): Biosynthesis and role in inflammatory resolution
    Lecture 5 (60 min + 15 min discussion) Cytochrome P450 derived eicosanoids and their biological implications: Biosynthesis and the role of soluble epoxide hydrolase as potential molecular drug target
    Lecture 6 (60 min + 15 min discussion) Endocannabinoids as multifunctional lipid mediators in health and disease: Biosynthesis, degradation and biological implications.
    Lecture 7 (60 min + 15 min discussion) Sphingolipids as structural membrane components and lipid mediators: Classification, biosynthesis, degradation and their role in health and disease.
    Lecture 8 (60 min + 15 min discussion) Diacylglycerols as second messangers in ligand-triggered intracellular signaling cascades: Biosynthesis, degradation and biological implications.
    Lecture 9 (45 min + 15 min discussion) Hepoxilins as inflammatory mediators and their involvement in epidermal differentiations
    Lecture 10 (60 min + 15 min discussion) Eicosanoid receptors, their intracellular signaling cascades and pharmacological interference.
    Lecture 11 (60 min + 15 min discussion) Evolutionary aspects of lipid signaling. Eicosanoid synthesizing enzymes and receptors in the three domains of terrestrial life and during mammalian evolution.
    Lecture 12 (60 min + 15 min discussion) Introduction to lipid signaling in plants- Biochemistry of Oxygenase (Lipoxygenase, hydroperoxide lyase and hydroperoxide cyclase) pathways leading to formation of hydroperoxides, jasmonic acid, traumatin, hexeno(a)ls, nonen(o)als etc
    Lecture 13 (60 min + 15 min discussion) Role of lipid signaling in the regulation of physiological processes- seed germination, growth and development, fruit ripening and senescence etc.
    Lecture 14 (60 min + 15 min discussion) Role of lipid signaling in plant defence responses- injury and infection, pathogen resistance/susceptibility etc.

    Module B: Lipid signaling in specific diseases

    Lecture 15 (45 min + 15 min discussion) Lipid signaling in inflammatory disorders
    Lecture 16 (45 min + 15 min discussion) Lipid signaling in cardio-vascular diesease (blood pressure regulation, atherogenesis and thrombosis)
    Lecture 17 (45 min + 15 min discussion) Lipid signaling in hyperproliferative diseases (cancer)
    Lecture 18 (45 min + 15 min discussion) Lipid signaling in neurodegeneration and cerebral ischemia
    Lecture 19 (45 min + 15 min discussion) Lipid signaling in obesity and other metabolic disorders (Diabetes mell.)
    Lecture 20 (45 min + 15 min discussion) Lipid signaling in infectious diseases
    Lecture 20 & 21 (45 min + 15 min discussion each) Lipid signaling in specific viral, baterial and fungal diseases in plants

    Module C: Tutorials and practicle courses

    Tutorial 1 (3 hours, introductory lecture plus practical course in small groups) Analytical strategies for lipid mediators: Activity assays for measuremnet of lipid signaling pathways in plants and animals-oxygraphic, spectrophotometric, ELISA etc
    Tutorial 2 (3 hours, introductory lecture plus practical course in small groups) Analytical strategies for lipid mediators: From immunoassays via HPLC to mass-spectrometry based lipidomics. The benefits and challenges of lipidomic methodologies.
    Tutorial 2 (3 hours, introductory lecture plus practical course in small groups) Oxidized phospholipids as markers of oxidative stress and as signaling molecules. Analytical strategies for quantification of the oxidized phospholipidome.
    Tutorial 3 (3 hours, introductory lecture plus practical course in small groups) In silico, in vitro and in vivo screening strategies for the search for inhibitors impacting the biosynthesis of lipid mediators
    Tutorial 4 (3 hours, introductory lecture plus practical course in small groups) In silico, in vitro and in vivo screening strategies for the search for target enzyme inhibitors and receptor antagonists of lipid mediators
    Tutorial 5 (3 hours, introductory lecture plus practical course in small groups) Analytical strategies for monitoring the lipid mediators during growth and developemnt, injury and infection in plant systems
    Note: If it is course work 3 internal exam will be conducted following final exam as presentations

    Module D: Closing session

    Round table discussion of the participants with all lecturers on various aspects of lipid signaling as well as on the analytical strategies