Workshop Write Up

Workshop Overview

Proteomics Facility, School of Life Sciences, University of Hyderabad (UoH) along with GE Fast Trak Training & Education, Bangalore (GE Healthcare Life Sciences) jointly invites you to a technology workshop on Large Scale Protein Purification Systems – a unique platform that showcases latest developments in downstream techniques with ÄKTA pilot and FlexStand Benchtop pilot systems. The 3-day workshop at UoH provides an exceptional hands-on learning experience for process development and manufacturing scientists, faculty, students, engineers, plant operators, and support staffs. During the three-day session, we will walk you through solutions which can enable you to intensify your downstream process. The three-day basic practical course covers downstream processing techniques suitable for production-scale protein purification and illustrates how these techniques should be considered for process development.

The focus of this course is on the understanding of the techniques and the parameters governing separation. Detailed presentations of the downstream processing techniques used (crossflow filtration & chromatography) are included as well as lectures covering column packing, purification strategies, column maintenance and process hygiene are briefly presented to describe the industrial environment and its principal objectives. During the practical session, you will work in small groups and use ÄKTA pilot and Flex stand filtration systems to perform the experiments.

Who can attend

♦  Researchers and Faculty at University, Government, and industrial laboratories.
♦  Students at all levels (MSc/PhD) from academic and technical institutions.

Registration :

Registration fee for academic Institutions/Students Rs.7500/-, for Industry Scientists Rs.15,000/- & Note: For UoH Students Registration fees Rs. 3000/-
The Demand Draft may be drawn in favour of "Finance Officer, University of Hyderabad" under PROTEOMICS USER CHARGES A/C no.10222816262.

After the course, you will be able to:

♠  Understand the critical issues in Lab and Pilot scale column packing based on your own practical experience.
♠  Pack and test Lab and Pilot scale columns more rapidly and efficiently.
♠  Identify major issues, trouble shoot current concerns and avoid problems in the future.
♠  Screen and optimize bioprocesses in your process development work.
♠  Understand the issues associated with optimizing chromatographic unit operations in biopharmaceutical production processes.
♠  Choose the optimum membrane format or technique based on target molecule and process objective.
♠  Define process conditions critical to the success of membrane applications.
♠  Evaluate experimental results for optimization and scale-up calculations.



Dr. Murali K.R.Tummuru is the Founder & Executive Director of Virchow Biotech Pvt. Ltd which is one of the largest producers of plasma derived products in India, with extensive global exports and has cGMP approved facilities that are recognized world wide including by the US-FDA and EU. As the executive director, Dr. Murali is involved in the development and commercialization of human plasma derived products and has an extensive publication history. He has published more than 30 Research papers in various international journals llike PNAS and JBC and has several US patents to his credit.


Distinguished Professor Emeritus, Penn State University, University Park, PA 16801. He obtained his PhD in Biochemistry from Indian Institute of Science, Bangalore, in 1975 and Joined as a Post-Doc in the department of Chemistry at Penn State University. He is currently Distinguished Professor Emeritus at Penn State. He was the Chairman of the Department of Veterinary and Biomedical Sciences from 1994 to 2006 at Penn State. Also, he served as the Director of the Huck Institutes of the Life Sciences from 2002 t0 2006 at Penn State. He was an awardee of “Research Career Development Award” (RCDA) from NIH (1983-1988). He was Elected Fellow of the American Association for the Advancement of Science in1992.
During his 35-year tenure, he served on 3 NIH study sections and also member of the Editorial Board for a number Scientific Journals. Recently, he has taken retirement and started a Biotech Company “INDUS Gene Expressions Ltd” near Bangalore. The Company’s main focus is contract manufacturing of Monoclonal Antibody vaccines.

Title of the talk: "Large-Scale Purification of Recombinant Proteins: Current Status and Future Trends"

There is an ever-increasing requirement for protein production in industrial and academic settings for a variety of applications. These include exploratory research, drug discovery initiatives, biopharmaceutical production, target validation, and high-throughput screening. With the advent of modern fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance. Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products. A comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings will be presented. Also discussed will be how innovative and effective protocols such as aqueous two-phase partitioning, membrane chromatography, field-assisted and electrophoretic separation methods, and high-performance tangential flow filtration may be applied independently of or in conjunction with more traditional protocols for downstream processing applications. The use of combinatorial chemistry and phage-display technologies is generating a large repertoire of ligands specific for virtually any protein. It is likely that these ligands will be increasingly used in future affinity chromatographic applications for large-scale recombinant protein purification.


Prof. Kambadur Muralidhar obtained his PhD from IISc, Bangalore in 1976 for his work demonstrating that beta subunit of Luteinizing Hormone can bind ovarian receptors. His post-doctoral work led to the development of the most sensitive RIA for hCG, a pregnancy hormone and establishment of the immuno-contraceptive vaccine potential of DS5-hCG beta subunit. He became Professor in 1988 at Delhi University. He initiated a long range research on water buffalo endocrinology, his lab was among the first to report that LH and PRL were sulphated in their carbohydrate. His lab later discovered the presence of Tyrosine-O-sulphate in sheep and buffalo PRL. His group has published over 100 peer-reviewed papers and articles. He is an outstanding teacher and has taught courses in Biochemistry, Gamete Biology, Immunology, Endocrinology and Cell Biology and Medicinal Chemistry. So far 26 PhDs and 27 MPhils have been awarded under his supervision besides training over 2000 MSc students. He has made significant contributions to the national education in Science working with UGC, NCERT and Delhi University. He is an elected Fellow of NASI, INSA and IASc.
He has published more than 145 articles in peer reviewed international and National Journals, Books and Monographs. He retired as Professor and HOD from Delhi University but continued as a JC Bose National Fellow at South Asian University, New Delhi. He has served all the academies, funding agencies, more than fifty Universities and research institutions in various capacities. Presently he is Jawaharlal Nehru Chair Professor at UoH.

Title of the talk: “Purification in Industry”.

Separation techniques are very essential in achieving biochemical understanding of biological/physiological phenomena/processes. Reductionist biology believes in analyzing complex systems by first breaking it into simpler systems and analyzing one at a time the simpler system. If a physiological process is mediated by ‘n’ number of enzymes/macromolecules, biochemists believe that each of the enzymes/macromolecules should be separated from others and studied in vitro before understanding the whole process. This necessitates the use of appropriate separation techniques. Obtaining a given enzyme/macromolecule, free from the others, in a homogeneous state is termed ‘purification’. Physical separation techniques like filtration, decantation, evaporation etc. only result in separating states of matter from each other i.e. solid from liquid, liquid from gas etc. Separation techniques to achieve separation of components of a molecular mixture (usually solid in solvent i.e. solutions) are based on exploitation of differences in physical properties (like charge density, solubility in water, size etc.) of the components of the mixture to achieve separation. For example, proteins differ from each other in either size (molecular mass), isoelectric point, net charge at a given pH of the solution, affinity to a small ligand or solubility in a salt solution. Precipitation (phase separation), chromatography, electrophoresis, differential adsorption etc. are some techniques used to achieve separation, exploiting the differences in the above mentioned physico-chemical properties of macromolecular components of a mixture. Biochemists therefore, use separation techniques most of their time. When these techniques are used in laboratory scale, for separating small quantities of biomolecules, they are usually called analytical techniques. On the other hand, if applied to large quantities (grams to kilograms) of biomolecular separations (i.e. industrial scale) they are termed as ‘downstream processes’. Separation techniques are applicable not only for the purification of functional proteins in native state from their natural sources (i.e. a tissue of plant or animal origin or from microbial source) but also recombinant proteins made in a large biomass, from either culture medium or cells that are artificially grown in fermenters and reactors. Biotech industries make large quantities of enzymes, hormones, vaccines, antibodies and many other functional proteins and peptides. Many of them are based on using rDNA techniques and are usually expressed in bacterial, insect, mammalian and plant cells in culture or even in intact animals and plants. The latter are designated transgenic animals or plants. The expressed recombinant protein becomes a biopharmaceutical only when purified from the source in homogeneous state and proven to possess biological activity at a given level of potency. Downstream processing involves judicious use of novel separation techniques like salt precipitations, ion-exchange chromatography, gel permeation chromatography, smart polymer based phase separations, membrane based separations, pseudo-affinity chromatography employing immobilized affinity ligands like textile dyes or even immobilized antibodies, differential adsorption and desorption etc. There are hundreds of chromatography materials or gels, affinity ligands etc. One has to understand the theory underlying the separation efficiency including yield. Hence the specific activity (in defined units/mg) of the final product is an important indicator of separation efficiency. Bioassays in vitro/in vivo are essential components of any purification strategy.


Title of the talk: "Protein Purification Strategies: Academia vs Industry"

Dr. Rajendar received his Ph.D. from Tohoku University (Japan) on Bioanalytical/Biological Chemistry. He later worked as postdoctoral research associate at University of Alabama at Birmingham (USA).
●    Dr. Rajendar is currently working as GM, R&D at Biological E. Limited and he is responsible for various functional areas in R&D which ranges from purification, analytical development to formulation development. Before joining Biological E. Limited, he worked with Indian Immunologicals, Intas Biopharmaceuticals and Dr. Reddy’s Laboratories Ltd.
●    Dr Rajendar was an editorial board member for American Journal of Analytical Chemistry and was a member of American Chemical Society and Biophysical Society. He authored several international publications and delivered talks in National and international conferences.
●    He has more than 10 years of industrial experience and his expertise includes, analytical and process development of Biopharmaceuticals (Therapeutics and Vaccines).

Resource Persons:


With 18 years of experience in the Bioprocess domain, Dinesh has the ability to optimize purification strategies for biological & non-biological origin molecules in continuous & batch mode chromatography. Presently, he is the Senior Bioprocess Product Manager with GE HEALTHCARE –Life Sciences.
●   Expertise in design-in the customer’s process at minimal cost.
●  Design the continuous chromatography process either based on productivity concept or media utilization capacity concept using pore diffusion model from break through curve.
●  Developed the synchronized continuous capture step and connected batch mode chromatographic steps in the Mab process and an unattended viral inactivation step using rocking platform under single software control.
●   Expertise in design in the production sites without any large volume tanks or with less volume tank/ bags using buffer management system and also in optimizing the elution strategy for Multimodal chromatography technique.
●   Optimize/Trouble shooting the purification process using Design of Experiments.


15 years of industrial experience in biopharma industries worked in various type of protein purification processes from lab to production scale.Currently handling projects and leading training & education for (India and Asia pacific region) GE life sciences Fast Trak,Bangalore, India.

Panel Discussion by Prof. Kambadur Muralidhar


Contact Us

Monica Kannan, Scientific Officer
G-20, Proteomics Facility,
School of Life Science,
University of Hyderabad,
Prof. C.R.Rao Road, Gachibowli,
Hyderabad, 500046, India
Venu Aitham,
Territory Sales Manager
GE Healthcare Life Sciences
Ph: +91 9849110304